GB/T 30664-2014Chemicals -- Amphibian metamorphosis assay (English PDF)

Foreword

This standard was drafted in accordance with GB/T 1.1-2009 given rules.

The Standards and Organisation for Economic Co-operation and Development (OECD) guidelines for testing of chemicals No.231 (2009 Nian) "amphibian metamorphosis Test Review Price "agreement (in English) technical content.

This standard is managed by the National Standardization Technical Committee chemicals dangerous (SAC/TC251) and focal points.

This standard was drafted. China Inspection and Quarantine Science Research Institute, Research Center for Eco-Environmental Sciences, Chinese Academy of.

1 Scope

This standard specifies the chemical test methods amphibian metamorphosis principle, the method described, test procedures, data and reports.

This standard applies to chemical testing amphibian metamorphosis.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein Member. For undated references, the latest edition (including any amendments) applies to this document.

Standard Guide ASTME729 with fish, macroinvertebrates and amphibians were tested material acute toxicity test (Standardguideforconductingacutetoxicitytestsontestmaterialswithfishes, macroinvertebrates, andamphibians) ASTME1439 Xenopus embryos Standard Guide teratogenicity test (FETAX) [Standardguideforconductingthe frogembryoteratogenesisassay-Xenopus (FETAX)] NieuwkoopPD, FaberJ Xenopus Growth Table (NormaltableofXenopuslaevis)

3 Abbreviations

The following abbreviations apply to this document.

AMA. amphibian metamorphosis test (amphibianmetamorphosisassay).

HPT. hypothalamic - pituitary - thyroid (hypothalamic-pituitary-thyroid).

SVL. mouth to cloaca length (snouttoventlength).

hCG. human chorionic gonadotropin (humanchorionicgonadotropin).

DO. Dissolved oxygen (dissolvedoxygen).

MTC. the maximum tolerated concentration (maximumtoleratedconcentration).

LC50. lethal concentration (lethalconcentration50).

TSH. thyroid stimulating hormone (thyroidstimulatinghormone).

HLL. hindlimb length (hindlimblength).

OECD. Organization for Economic Cooperation and Development (organisationforeconomicco-operationanddevelopment).

NF. NieuwkoopPD, FaberJ (1994) on the normal development of Xenopus laevis morphological criteria developmental stage classification (Nieuwk-oopandFaber).

4 Principle of the method

4.1 AMA is a screening test, its purpose is to empirically identify possible substances interfere with the normal function of the HPT axis. In HPT axis Paul Keep within the scope of the structure and function, AMA represents a generalized vertebrate model approach. This is an important trial because two Amphibians metamorphosis is a study of more mature, thyroid-dependent process that HPT axis of the active substance within the responsive and this is currently the only active thyroid test animals detect morphological development.