GB/T 43241-2023Forensic sciences—Examination methods for nitrous oxide—Gas chromatography-mass spectrometry (English PDF)

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document describes the headspace gas chromatography-mass spectrometry (HS/GC-MS) detection of suspect gases and nitrous oxide in blood in the context of forensic science. test method.

This document is applicable to the qualitative analysis of nitrous oxide in suspicious gases and the qualitative analysis of nitrous oxide in blood in the field of forensic science and Quantitative analysis.

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document.

GA/T 122 Toxicological analysis terminology

3 Terms and definitions

The terms and definitions defined in GA/T 122 apply to this document.

4 Principles

For gases suspected of being nitrous oxide (see Appendix A for basic information on the compound), air and standard samples were used as controls, and headspace gas chromatography was used.

Spectrum-mass spectrometry detection, qualitative analysis based on retention time, mass spectrum characteristic ions and ion abundance ratio as the basis for qualitative judgment.

For nitrous oxide in blood, blank samples and added samples were used as controls, and headspace gas chromatography-mass spectrometry was used to detect the retention time.

Characteristic ions and ion abundance ratios of inter-mass spectrum and ion abundance ratio were used as the basis for qualitative judgment for qualitative analysis; chromatographic peak area was used as the quantitative basis, and external standard-single Point calibration method or external standard-calibration curve method was used for quantitative analysis.

5 Reagents and materials