GB/T 42821-2023Diagnostic methods for bonamiosis of molluscs (English PDF)

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document gives the abbreviations, reagents and materials, instruments and equipment for the diagnosis of shellfish bonamiosis, and describes the clinical Symptoms, sampling, tissue print examination, histopathological examination, PCR detection, fluorescent PCR detection and comprehensive judgment methods.

This document applies to shellfish encrustation caused by Bonamiaostreae and Bonamiaexitiosa Epidemiological investigation, diagnosis, quarantine and surveillance of nanodiseases.

2 Normative reference documents

The contents of the following documents constitute essential provisions of this document through normative references in the text. Among them, the dated quotations For undated referenced documents, only the version corresponding to that date applies to this document; for undated referenced documents, the latest version (including all amendments) applies to this document.

GB/T 6682 Specifications and test methods for water used in analytical laboratories

GB/T 18088 Entry and Exit Animal Quarantine Sampling

GB/T 19495.2 Technical requirements for genetically modified product testing laboratories

3 Terms and Definitions

There are no terms or definitions to be defined in this document.

4 Abbreviations

The following abbreviations apply to this document. bp. base pair (basepair)

Ct. amplification cycle number (cyclethreshold)

FAM. carboxyfluorescein Taq. Thermusaquaticus TE. Tris hydrochloric acid and EDTA buffer solution (EDTATris·HCl)

5 Reagents and materials

All chemical reagents in this document, unless otherwise specified, use only analytically pure reagents.