GB/T 41716-2022Determination of main active ingredient in lacquer trees—High preformance liquid chromatography (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting.

1 Scope

This document describes a method for the determination of the main active ingredients in sumac by high performance liquid chromatography.

This document is applicable to urushiol (saturated urushiol, monounsaturated urushiol, diunsaturated urushiol, triunsaturated and urushiol) and flavonoids (flavonoids, fisetin, thiamethrin and buterin) compounds for the determination of single or multiple components.

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

GB/T 36055 Determination of Moisture Content in Analytical Methods of Forestry Biomass Raw Materials

3 Terms and Definitions

The following terms and definitions apply to this document.

4 Principles

In the same analysis cycle, adjust the mobile phase ratio according to a certain time program, so that the main active components in sumac can be adjusted according to their respective appropriate capacities.

Factors achieve good separation purposes. Using saturated urushiol, monounsaturated urushiol, diunsaturated urushiol, triunsaturated urushiol, yellow pigment lignin, lacquer Flavin, thiochrysin and butacein have maximum ultraviolet absorption at specific wavelengths, and are detected under the conditions of corresponding wavelengths, and are tested with standard substances. Direct quantitative analysis by external standard method.