GB/T 40226-2021Detection of environmental microbial metagenome—High throughput sequencing (English PDF)
环境微生物宏基因组检测 高通量测序法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
May 21, 2021
Implementation date
December 1, 2021
Scope
GB/T 40226-2021 is the English-translated version of 环境微生物宏基因组检测 高通量测序法.
GB/T 40226-2021 describes a high-throughput sequencing method for detecting the metagenome of environmental microbial communities, meaning the DNA of all the microorganisms in a given environment, extracted directly without isolating or culturing them.
It covers the terms used, the principle, test conditions, reagents, instruments and equipment, samples, the test steps, the test report and quality control.
Normative annexes set out how stool, soil and water samples are collected, preserved and transported, while informative annexes give a sample information sheet, the DNA integrity and quality grades and reference databases.
Environments range from the human and animal body, such as the gut, mouth and skin, to air, water, soil and sediment. It was issued on 21 May 2021 and has applied since 1 December 2021.
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Document preview — GB/T 40226-2021
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- A 40
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Abbreviations
- 5 Principle
- 6 Test condition
- 7 Reagent
- 8 Instruments and equipment
- 9 Sample
- 10 Test Step
- 11 Test report
- 12 Quality Control
- Appendix A (normative) Methods of collecting, storing, and transporting stool samples
- Appendix B (Normative) Methods of soil sample collection, preservation, and transportation
- Appendix C (Normative) Methods of Water Sample Collection, Preservation, and Transportation
- Appendix D (Informative) Sample Information Sheet
- Appendix E (Informative) The completeness map and quality level classification of DNA samples
- Appendix F (Informative) Reference Database
- Reference
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.
1 Scope
This document describes the terms and definitions, principles, test conditions, Reagents, equipment, samples, test procedures, test reports and quality control requirements.
This document is suitable for the detection of environmental microbial metagenomics using high-throughput sequencing methods.
2 Normative references
The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.
GB/T 6682 Analytical laboratory water specifications and test methods
GB 19489 Laboratory Biosafety General Requirements
GB/T 35537-2017 High-throughput gene sequencing results evaluation requirements
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 Environment
The surrounding world relative to a central thing.
Note: In this document, it refers specifically to the human (animal) environment and the natural environment. The human (animal) environment includes but is not limited to the oral cavity, skin, reproductive tract, intestinal tract, etc.; natural The environment includes but is not limited to air, water, soil, sediment, etc.
3.2 Relative abundance
The relative proportion of a specific kind of microorganisms in the environmental microbial community.
Note: Usually expressed as a percentage.
3.3 Microbial metagenome
Take the entire microbial community in a specific environment as the research object, without separation and cultivation, directly extract all the microbial genes obtained Group DNA.
Note: It can be used to analyze its genetic information, species classification, system evolution, gene function and metabolic network, etc.
3.4 Quality of base calling
Evaluate the probability of accurate base recognition.
Note: Abbreviated as Q, usually expressed as a numerical value. The base recognition quality value is negatively correlated with the base recognition error rate, and the two follow a logarithmic function relationship. Base recognition quality The higher the value, the lower the error rate.
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Normative references
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Related Standards
GB 19489-2008 — Laboratories - General requirements for biosafety
GB/T 35537-2017 — Requirements of the high-throughput gene sequencing result evaluation
GB/T 6682-2008 — Water for analytical laboratory use - Specification and test methods
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