GB/T 35537-2017Requirements of the high-throughput gene sequencing result evaluation (English PDF)
高通量基因测序结果评价要求
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 29, 2017
Implementation date
July 1, 2018
Scope
GB/T 35537-2017 is the English-translated version of 高通量基因测序结果评价要求.
GB/T 35537-2017 is the Chinese standard "Requirements of the high-throughput gene sequencing result evaluation".
Its scope clause reads: This standard specifies the terminology and definitions involved in the evaluation of high-throughput gene sequencing results evaluation of high-throughput gene sequencing results evaluation Indicators and judgments.
This standard applies to DNA sequencing based on non-single-molecule sequencing of ligase sequencing and polymerase sequencing of the results of evaluation. Its clauses include terms and definitions and abbreviations.
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Document preview — GB/T 35537-2017
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- A 40
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and definitions and abbreviations
- 3.1 Terms and definitions
1 Scope
This standard specifies the terminology and definitions involved in the evaluation of high-throughput gene sequencing results evaluation of high-throughput gene sequencing results evaluation
Indicators and judgments.
This standard applies to DNA sequencing based on non-single-molecule sequencing of ligase sequencing and polymerase sequencing of the results of evaluation.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 30989 high throughput gene sequencing technical specifications
3 Terms and definitions and abbreviations
3.1 Terms and definitions
GB/T 30989 defined and the following terms and definitions apply to this document.
3.1.1 High-throughput gene sequencing results
The data produced by the sequencer is also known as the sequencing result.
3.1.2 The average length of sequencing
The average read length that a sequencing instrument can achieve in a single run.
Note. usually expressed in terms of bases.
3.1.3 Sequencing throughput
The number of sequences available to a sequencer in a single run.
Note. Usually expressed as a sequence number (Reads).
3.1.4 Base sequencing accuracy
The reference sequence of the known sequence is sequenced, compared to the known sequence after recognition of the base, and the number of the correct base of the statistical alignment is accounted for by sequencing
The total number of bases obtained.
3.1.5 Sequencing by polymerase
Based on the principle of base complementation, gene sequencing method with polymerase involved.
3.1.6 Ligase sequencing
Based on the principle of base complementation, gene sequencing method with the involvement of ligase.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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