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GB/T 40171-2021General rules for determination of magnetic bead DNA extraction and purification kit (English PDF)

磁珠法DNA提取纯化试剂盒检测通则

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

May 21, 2021

Implementation date

December 1, 2021

Scope

GB/T 40171-2021 is the English-translated version of 磁珠法DNA提取纯化试剂盒检测通则.

GB/T 40171-2021 sets general rules for testing magnetic bead DNA extraction and purification kits.

It describes the test principle, the preparation before testing and the test methods for the magnetic beads themselves, DNA extraction and purification, DNA concentration and purity, DNA yield and integrity, the adsorption rate of the magnetic beads, the extraction recovery rate and precision, followed by the test report.

It applies to kits used on a wide range of biological samples: plant and animal tissues, blood, blood spots and clots, plasma, saliva, semen, urine, feces and other secretions, bacteria, viruses and fungi.

Its terms define magnetic beads and their adsorption rate, the ratio of nucleic acid adsorbed by the beads to the total amount of nucleic acid. It was issued on 21 May 2021 and has applied since 1 December 2021.

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Document preview — GB/T 40171-2021

National Standard of the People's Republic of China

ICS: 07.080
Classification: A 40

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Foreword
1 Scope
2 Normative references
3 Terms and definitions

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.

1 Scope

This document specifies the general rules for the detection of magnetic bead DNA extraction and purification kits, and describes the detection principle, preparation before detection, and the detection of magnetic beads.

Method DNA extraction and purification kit magnetomechanical properties, DNA extraction and purification, DNA concentration and purity, DNA yield, DNA integrity, magnetism Bead adsorption rate, extraction recovery rate, precision detection method and test report.

This document applies to plant tissues, animal tissues, blood, blood spots, blood clots, plasma, etc., saliva, semen, urine, feces and other secretions, fine Determination of magnetic bead DNA extraction kits in biological samples such as bacteria, viruses, and fungi.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB 5009.268 National Food Safety Standard Determination of Multi-elements in Food

GB/T 19077 Particle Size Analysis Laser Diffraction Method

GB/T 21649.1 Particle Size Analysis Image Analysis Method Part 1: Static Image Analysis Method

GB/T 29022 Particle Size Analysis Dynamic Light Scattering Method (DLS)

GB/T 34794 Agarose gel recovery kit determination general rule

GB/T 34796 Determination of the concentration and purity of nucleic acids in aqueous solutions by ultraviolet spectrophotometry

SN/T 2775 Evaluation Method of Commercial Food Testing Kit

3 Terms and definitions

The following terms and definitions defined in GB/T 34794 and GB/T 34796 apply to this document.

3.1 Magnetic beads

A functionalized carrier whose surface has been functionally modified and coated with biologically active groups can quickly accumulate in a magnetic field and leave the magnetic field. After the field, it can quickly disperse, and realize the specific and efficient binding and dissociation of nucleic acid molecules under different conditions with superparamagnetism. Nano or micron spherical or amorphous particles.

Note 1. Its surface modification has a wealth of active groups, which can be reversibly combined with nucleic acid molecules under certain buffer conditions.

Note 2. Compared with traditional separation methods, magnetic beads are used for the separation of complex components of biochemical samples, which can achieve separation and enrichment at the same time, which effectively improves the separation. The separation speed and enrichment efficiency also greatly improve the sensitivity of analysis and detection.

Note 3. Magnetic beads in this document refer to biological separation magnetic beads. This kind of magnetic beads has superparamagnetism and can be dispersed in the base fluid to form magnetic liquid materials and biologically active groups It can be coupled with a variety of biologically active substances, has the dual characteristics of liquid fluidity and solid magnetic particle materials, and has a definite particle size Scope, there are certain surface characteristics, including specific surface area, surface modification, etc.

3.2 Adsorption rate of magnetic beads

Under certain conditions, the ratio of nucleic acid adsorbed by magnetic beads to the total amount of nucleic acid.

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Referenced standards

Normative references

Similar standards

GB/T 40980-2026|GB/T 47142-2026|GB/T 47145-2026|GB/T 47199-2026|GB/T 47201-2026|GB/T 47232-2026

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