GB/T 34794-2017Guidelines for DNA gel purification kit (English PDF)
琼脂糖凝胶回收试剂盒测定通则
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 1, 2017
Implementation date
May 1, 2018
Scope
GB/T 34794-2017 is the English-translated version of 琼脂糖凝胶回收试剂盒测定通则.
GB/T 34794-2017 is the Chinese standard "Guidelines for DNA gel purification kit". Its scope clause reads: This standard specifies the column agarose gel recovery kit DNA recovery product evaluation indicators and test methods.
This standard applies to column agarose gel recovery kit determination. Its clauses include terms and definitions; principle.
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Document preview — GB/T 34794-2017
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- G 66
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 principle
1 Scope
This standard specifies the column agarose gel recovery kit DNA recovery product evaluation indicators and test methods.
This standard applies to column agarose gel recovery kit determination.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version applies to this article
Pieces. For undated references, the latest edition (including all amendments) applies to this document.
GB/T 6682 analytical laboratory water specifications and test methods
GB/T 11165 Laboratory pH meter
GB/T 26813 dual-beam UV-visible spectrophotometer
JJG646 pipette test procedures
YY/T 0087 electrophoresis device
YY/T 0657 medical centrifuge
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 DNA purification
Using appropriate methods to reduce observable or measurable processes of ligation, cleavage, labeling, amplification and other inhibitors, to obtain higher
Purity DNA.
3.2 DNA recovery DNArecoveryrate
That is, the recovery rate of DNA, the percentage of yield after DNA target fragment recovery and before recovery.
3.3 The maximum sample volume columnreservoircapacity
A single adsorption column of a single maximum loading volume.
3.4 Loading capacity of adsorption column
A single adsorption column can absorb up to the amount of DNA each time. It determines the maximum amount of DNA recovered in a single shot.
4 principle
Media adsorption is a commonly used method of purifying nucleic acids. Column Recycling Kit is a silica gel membrane or other adsorption medium fixed in the distance
As the adsorbent on the column of heart, under the condition of high salt (such as 3.0mol/L ~ 5.0mol/L guanidine hydrochloride) low pH (5.0mol/L ~ 6.5mol/L) […]
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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