GB/T 37272-2018Determination of 11-nor-9-carboxy-delta-9-tetrahydrocannabinol in urine—Liquid chromatography-tandem mass spectrometry method (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for the determination of delta9-tetrahydrocannamic acid in urine.

This standard applies to the qualitative analysis and quantitative analysis of delta9-tetrahydrocannamic acid in urine.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

GA/T 122 Toxic Analysis Terminology

3 Terms and definitions

The terms and definitions defined by GA/T 122 apply to this document.

4 Principle

In the urine, delta9-tetrahydrocannanic acid is hydrolyzed under alkaline conditions, and then extracted under acidic conditions with an organic solvent, using liquid chromatography-tandem Mass spectrometry was performed.

5 Reagents or materials

The reagents used are analytically pure reagents unless otherwise specified; water is primary water in accordance with GB/T 6682; reagents and materials include.

a) n-hexane.

b) Ethyl acetate.

c) Acetonitrile. HPLC grade.

d) Formic acid. excellent grade.

e) Ammonium acetate. chromatographically pure.

f) Glacial acetic acid. excellent grade pure.

g) 20mmol/L ammonium acetate solution. Take 1.54g of ammonium acetate, put it in a 1000mL volumetric flask, add appropriate amount of water, add 2.0mL of formic acid, Add water to the mark and shake well.

h) 1mol/L sodium hydroxide solution. Take 4.0g of sodium hydroxide and dissolve it with 100mL of water.

i) 1mol/L hydrochloric acid solution. Take 20.8mL of concentrated hydrochloric acid and dilute to 250mL with water.

j) delta9-tetrahydrocannabinic acid reference solution. a commercially available 100 µg/mL methanol solution, stored in a freezer in a refrigerator. Save time is 12 months.