GB/T 36191-2018Protocol of diagnostic methods for red sea bream iridovirus disease (RSIVD) (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the clinical diagnosis, sampling and virus of Redseabreamiridovirus disease (RSIVD). Separation and identification and comprehensive determination methods.

This standard applies to the epidemiological investigation, diagnosis, quarantine and monitoring of aquatic animals.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and experimental methods

GB/T 18088 entry and exit animal quarantine sampling

SC/T 7103 Technical specification for quarantine sampling of aquatic animals

3 Abbreviations

The following abbreviations apply to this document.

BLAST. Local Sequence Alignment Search Tool (basiclocalalignmentsearchtool)

Bp. basepair

CTAB. cetyltrimethylammonium bromide

CPE. cytopathic effect

DMEM. DulbecoEagle'sminimumessentialmedium

EB. ethidium bromide (ethidiumbromide)

FITC. fluorescein isothiocyanate

IFAT. Indirect fluorescent antibody test (indirectfluorescent antibody test)

ISKNV. infectious spleen and kidney necrosis virus (infectiousspleenandkidneynecrosisvirus)

MFF-1. carp fry cell line (mandarinfishfrycelline)

PBS. phosphate buffered saline (phosphatebufferedsaline)

PBST. pH 7.4 phosphate buffer (0.05% tween phosphate bufferedsaline) containing 0.05% Tween-20

PCR. polymerase chain reaction (polymerase chain reaction)

RSIV. true red iris virus (redseabreamiridoviralvirus)

RSIVD. true red iris virus (redseabreamiridoviraldisease)

4 Reagents and materials

4.1 Water. Meet the specifications of the first grade water in GB/T 6682.