GB/T 36190-2018Protocol of diagnostic methods for hemorrhagic disease of grass carp (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the clinical symptoms, sampling, virus isolation and identification, and comprehensive judgment of grass carp bleeding disease diagnosis.

This standard applies to epidemiological investigation, diagnosis, quarantine and monitoring of grass carp hemorrhagic disease.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document.

For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

GB/T 18088 entry and exit animal quarantine sampling

SC/T 7016.3 Fish Cell Lines Part 3. Grass carp ovary cell line (CO)

SC/T 7103 Technical specification for quarantine sampling of aquatic animals

3 Abbreviations

The following abbreviations apply to this document.

AMV. avian myeloblastosis virus (avian myeloblastosis virus)

Bp. basepair

cDNA. Complementary deoxyribonucleic acid (complementarydeoxyribonucleicacid)

CO. grass carp ovary cell line (grasscarpovarycelline)

CPE. cytopathic effect

CTAB. cetyltrimethylammonium bromide

dNTP. deoxy-ribonucleoside triphosphate

dATP. deoxyadenosine triphosphate (deoxyadenosinetriphosphate)

dCTP. deoxycytidine triphosphate (deoxycytidinetriphosphate)

dGTP. deoxyguannosine triphosphate (deoxyaguanosinetriphosphate)

dTTP. deoxythymidine triphosphate (deoxythymidinetriphosphate)

EB. ethidium bromide (ethidiumbromide)

EDTA. ethylenediaminetetraacetic acid

ELISA. enzyme-linked immunosorbent assay (enzyme-linked immunosorbent assay)

FBS. fetal bovine serum (fetalbovineserum)

GCRV. grass carp reovirus (grasscarpreovirus)

HEPES. 4-hydroxyethylpiperazineethanesulfonic acid [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid]

HRP. horseradish peroxidase (horseradishperoxidase)

MCP. major capsid protein (majorcapsidprotein)

PBST. phosphate buffer solution (phosphatebuffersolution-tween-20)

RT-PCR. reverse transcription-polymerase chain reaction (reverse transcription polymerase chain reaction)