GB/T 35911-2018Real-time PCR method for detection of pseudorabies virus (English PDF)

Foreword

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This standard specifies the method for the detection of pseudorabies virus fluorescent PCR.

This standard applies to the detection of pseudorabies virus nucleic acid.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this document. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

GB/T 25172 Pig normal temperature semen production and preservation technical specifications

3 Abbreviations

The following abbreviations apply to this document.

Fluorescent PCR fluorescent polymerase chain reaction (real-time polymerase chain reaction)

Ct value Cycle number (cyclethreshold) experienced when the amount of fluorescent signal in each reaction tube reaches a set threshold

DNA deoxyribonucleic acid (deoxyribonucleic acid)

Taq enzyme Taq DNA polymerase (TaqDNApolymerase)

TE buffer Tris-EDTA buffer (Tris-EDTAbuffer)

PRV pseudorabies virus (pseudorabiesvirus)

4 Instruments

4.1 Fluorescence PCR detector.

4.2 High-speed desktop refrigerated centrifuge. can control temperature to 4 ° C, centrifugal speed can reach 12000r/min or more.

4.3 Tissue grinder or mortar.

4.4 Ordinary refrigerator. 2 ° C ~ 8 ° C.

4.5 Ordinary freezer. below -20 °C.

4.6 Ultra-low temperature refrigerator. can control temperature below -70 °C.

4.7 Micropipette. 0.2µL~2µL, 1µL~10µL, 10µL~100µL, 20µL~200µL, 100µL~1000µL, and Comes with a tip that matches the pipette.

4.8 Autoclave.

5 Supplies

5.1 1.5 mL centrifuge tube.

5.2 0.2mL PCR thin wall tube or eight tube.