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GB/T 34265-2017Guide for the method of Sanger sequencing (English PDF)

Sanger法测序技术指南

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

September 7, 2017

Implementation date

April 1, 2018

Scope

GB/T 34265-2017 is the English-translated version of Sanger法测序技术指南.

GB/T 34265-2017 is the Chinese standard "Guide for the method of Sanger sequencing". Its scope clause reads: This standard specifies the terms and definitions Sanger sequencing method, abbreviations, principles, major equipment, equipment, Sanger gene sequencing Index, Sanger gene sequencing method, Sanger method gene sequencing applications.

This standard applies to animal tissues, plant tissues, blood, oral mucosa, hair, feces, soil, microorganisms and other biological samples DNA was sequenced by the Sanger sequencing method. Its clauses include terms and definitions.

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Document preview — GB/T 34265-2017

National Standard of the People's Republic of China

ICS: 07.080
Classification: A 40

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Preface
Introduction
1 Scope
2 Normative references
3 Terms and definitions
4 Abbreviations
5 principle
6 major equipment, equipment
7 Sanger gene sequencing index
8 Sanger gene sequencing method
9 Sanger method gene sequencing applications
Appendix A (informative) Sanger method sequencing principle diagram
Appendix B (informative) reagents and solution preparation
Appendix C (informative) Preparation of sequencing reaction system
Appendix D (informative) amplification program
Appendix E (Informative) to be sequenced product purification
References

1 Scope

This standard specifies the terms and definitions Sanger sequencing method, abbreviations, principles, major equipment, equipment, Sanger gene sequencing

Index, Sanger gene sequencing method, Sanger method gene sequencing applications.

This standard applies to animal tissues, plant tissues, blood, oral mucosa, hair, feces, soil, microorganisms and other biological samples

DNA was sequenced by the Sanger sequencing method.

2 Normative references

The following documents for the application of this document is essential. For dated references, only the dated version applies to this article

Pieces. For undated references, the latest edition (including all amendments) applies to this document.

GB/T 6682 analytical laboratory water specifications and test methods

3 Terms and definitions

The following terms and definitions apply to this document.

3.1 Gene sequencing

The determination of the nucleotide sequence of the nucleic acid molecules, that is, the determination of adenine (A), guanine (G), cytosine (C)

And thymine (T) or uracil (U) in the order of arrangement.

[GB/T 30989-2014, Definition 3.18]

3.2 Sanger sequencing method

The dideoxy chain termination method used for gene sequencing is randomly blocked during the chain extension with a fluorescently labeled dideoxy base to give

A, T, C, G end of the four groups of different lengths of nucleotide chain, by reading the fluorescent signal to achieve nucleic acid base sequence information read.

3.3 Denaturation

The secondary or tertiary structure of a protein or nucleic acid is altered by physical or chemical factors leading to changes in its physico-chemical properties and biological activity

Sexual loss phenomenon.

3.4 Primer

In the DNA replication process, the start site and/or termination site bound to the template strand and extending as a replication has a length

Degree and order of oligonucleotide chains.

[GB/T 30989-2014, Definition 3.11]

3.5 Readlengthofgenesequencing

The length of the longest gene fragment that can be sequenced.

Note. usually expressed in terms of bases.

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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