GB/T 40170-2021Principle of plasmid extraction and testing (English PDF)
质粒抽提及检测通则
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
May 21, 2021
Implementation date
December 1, 2021
Scope
GB/T 40170-2021 is the English-translated version of 质粒抽提及检测通则.
GB/T 40170-2021 lays down general rules for extracting plasmids and testing them. It covers terms and abbreviations, principles, the extraction itself, testing, quality control, sample preservation, reporting and the disposal of waste.
The definitions include plasmid, ribonuclease A and bacterial endotoxin, and the standard draws on referenced methods such as ultraviolet spectrophotometry for the concentration and purity of nucleic acids in aqueous solution and Sanger sequencing, together with the requirements for genetically modified product testing laboratories and the quality control of molecular biology testing.
It applies to the extraction and testing of plasmids in general. It was issued on 21 May 2021 and has applied since 1 December 2021.
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Document preview — GB/T 40170-2021
National Standard of the People's Republic of China
- ICS
- 07.080
- Classification
- A 40
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Foreword
- 1 Scope
- 2 Normative references
- 3 Terms and definitions
- 4 Abbreviations
Foreword
This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules of Standardization Documents" Drafting.
1 Scope
This document specifies the terms and definitions, abbreviations, principles, extraction, testing, quality control, sample preservation, and reporting of plasmid extraction and testing And waste disposal.
This document is suitable for the extraction and testing of plasmids.
2 Normative references
The contents of the following documents constitute indispensable clauses of the documents through normative references in the text. Among them, the dated reference documents, only The version corresponding to this date is applicable to this document; for undated references, the latest version (including all amendments) is applicable to this document.
GB 4789.3 National Food Safety Standard Food Microbiological Inspection Coliform Count
GB/T 19495.1 General requirements and definitions for the testing of genetically modified products
GB/T 19495.2 Technical requirements for genetically modified products testing laboratories
GB/T 27403 Laboratory Quality Control Specification Food Molecular Biology Testing
GB/T 34265 Sanger method sequencing technology guide
GB/T 34796 Determination of the concentration and purity of nucleic acids in aqueous solutions by ultraviolet spectrophotometry
3 Terms and definitions
The following terms and definitions apply to this document.
3.1 Plasmid
One or more closed circular double-stranded DNA molecules other than chromosomes (or nucleoids) in organisms such as bacteria, yeasts, and actinomycetes.
Note: It exists in the cytoplasm and has the ability to replicate independently, enabling it to maintain a constant copy number in the progeny cells and express the genetic information it carries.
3.2 Ribonuclease A
A kind of 3'and 5'phosphodiester bonds of the ribose moiety that catalyzes the hydrolysis of ribonucleic acid to form oligonucleosides with 2', 3'-cyclic monophosphate derivatives Acid nuclease.
3.3 Bacterial endotoxin
A complex composed of hydrophilic heteropolysaccharides and a covalently bound lipid A.
Note: The complex is located on the cell wall of gram-negative bacteria. Suction distress, intravascular coagulation and endotoxin shock syndrome.
4 Abbreviations
The following abbreviations apply to this document.
DNA. Deoxyribonucleic Acid (Deoxyribonucleic Acid)
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Referenced standards
Normative references
- GB 4789.3National food safety standard - Food microbiological examination: Enumeration of coliforms
- GB/T 19495.1Detection of genetically modified organisms and derived products--General requirements and definitions
- GB/T 19495.2Detection of genetically modified organisms and derived products--General requirements for laboratories
- GB/T 27403Criterion on the quality control of laboratories — Molecular biological testing of food
- GB/T 34265Guide for the method of Sanger sequencing
- GB/T 34796Quantification and purity analysis of nucleic acid concentration in solution - Ultraviolet spectrophotometry
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Related Standards
GB 4789.3-2025 — National food safety standard - Food microbiological examination: Enumeration of coliforms
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