Valid

GB/T 32946-2016Determination of proline in honey - High performance liquid chromatography (English PDF)

蜂蜜中脯氨酸的测定高效液相色谱法

Preview PDF

This is a limited preview

Buy now to download the full PDF (15 pages)

Need quotation for your management?

Get a formal quotation for GB/T 32946-2016 in 30 minutes.

Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

August 29, 2016

Implementation date

March 1, 2017

Scope

GB/T 32946-2016 is the English-translated version of 蜂蜜中脯氨酸的测定高效液相色谱法.

GB/T 32946-2016 is the Chinese standard "Determination of proline in honey - High performance liquid chromatography". Its scope clause reads: This standard specifies the content of proline in honey by high performance liquid chromatography.

This standard applies to the determination of proline content in honey. Its clauses include normative reference documents; method summary; reagents and materials.

Found what you need?

Document preview — GB/T 32946-2016

National Standard of the People's Republic of China

ICS: 67.180.10
Classification: X 31

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

1 Scope
2 normative reference documents
3 method summary
4 reagents and materials

1 Scope

This standard specifies the content of proline in honey by high performance liquid chromatography.

This standard applies to the determination of proline content in honey.

2 normative reference documents

The following documents are indispensable for the application of this document. For dated references, only the dated edition applies to this article

Pieces. For undated references, the latest edition (including all modifications) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods

3 method summary

The proline in the sample was extracted with boric acid buffer, derivatized by column, liquid chromatographic fluorescence detector, external standard method.

4 reagents and materials

4.1

Unless otherwise stated, analytical reagents are used and water is the primary water specified in GB/T 6682.

4.2 Methanol

Chromatographic purity.

4.3 acetonitrile

pure chromatography.

4.4 tetrahydrofuran

chromatographic purity.

4.5

Sodium acetate.

4.6

Acetic acid.

4.7

boric acid.

4.8

disodium ethylenediaminetetraacetate.

4.9

Sodium hydroxide.

4.10

phosphoric acid.

4.11

hydrochloric acid.

4.12 L-proline standard (CAS number

147-85-3). purity >=99%.

4.14

sodium hydroxide solution (0.1mol/L). Weigh 2.00g sodium hydroxide, dissolved in 500mL of water, mix, transfer to the polyethylene bottle,

Closed place.

4.15

Sodium acetate buffer (0.1 mol/L). Weigh 13.60 g of sodium acetate, dissolve in 1 L of water, adjust the pH to 7.2 with acetic acid,

Degassed.

4.16

boric acid buffer (0.1mol/L). Weigh ethylenediamine tetraacetic acid disodium 0.186g, boric acid 3.09g, dissolved in 500mL water, hydrogen

Sodium hydroxide solution adjusted to pH 8.0.

4.17

hydrochloric acid solution (0.1mol/L). take 8.33mL hydrochloric acid, constant volume to 1L.

4.18 NBD-F solution (100mmoL/L)

Weigh NBD-F0.0183g in a brown liquid phase vial, add 1mL acetonitrile dissolved, […]

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

Similar standards

GB/T 47417-2026|GB/T 47735-2026|GB/T 44350-2024|GB/T 43448-2023|GB/T 40152-2021|GB/T 33045-2016

How to Download GB/T 32946-2016

1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.