GB/T 32262-2015Preparation of deoxyribonucleic acid sample for atomic force microscope measurement (English PDF)
用于原子力显微镜检测的脱氧核糖核酸样品的制备方法
This is a limited preview
Buy now to download the full PDF (15 pages)
Need quotation for your management?
Get a formal quotation for GB/T 32262-2015 in 30 minutes.
Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 10, 2015
Implementation date
January 1, 2017
Scope
GB/T 32262-2015 is the English-translated version of 用于原子力显微镜检测的脱氧核糖核酸样品的制备方法.
GB/T 32262-2015 is the Chinese standard "Preparation of deoxyribonucleic acid sample for atomic force microscope measurement". Its scope clause reads: This standard specifies a method for preparing for AFM detection and analysis of DNA samples, including.
Summary of methods, experimental materials and devices Wood preservation, environmental conditions, modified mica substrate, a solution of DNA processing, fixed on the substrate DNA, DNA samples, DNA samples The evaluation.
This standard applies to the preparation of a single DNA molecule by AFM high resolution imaging and nano-manipulation experiments in atomic adsorption stage Various DNA samples on flat mica substrate, and to study the physical and chemical properties and biological functions.
Similar to the molecular structure of DNA Fixed biological material can also refer to this standard. Its clauses include terms and definitions, abbreviations.
Found what you need?
Document preview — GB/T 32262-2015
National Standard of the People's Republic of China
- ICS
- 07.030
- Classification
- A 40
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- Introduction
- 1 Scope
- 2 Normative references
- 3 Terms and definitions, abbreviations
- 3.1 Terms and definitions
- 3.2 Abbreviations
- 4 Summary of Method
- 5 experimental materials and equipment
- 6 environmental conditions
- 7 DNA sample preparation procedures
- 7.1 Modified mica substrate
- 7.2 DNA solution was treated
- 7.3 DNA immobilized on the substrate
- 8.1 Detection
- 8.2 Evaluation
1 Scope
This standard specifies a method for preparing for AFM detection and analysis of DNA samples, including. Summary of methods, experimental materials and devices
Wood preservation, environmental conditions, modified mica substrate, a solution of DNA processing, fixed on the substrate DNA, DNA samples, DNA samples
The evaluation.
This standard applies to the preparation of a single DNA molecule by AFM high resolution imaging and nano-manipulation experiments in atomic adsorption stage
Various DNA samples on flat mica substrate, and to study the physical and chemical properties and biological functions. Similar to the molecular structure of DNA Fixed biological material can also refer to this standard.
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
Laboratory use specifications and test methods GB/T 6682-2008 Analysis
3 Terms and definitions, abbreviations
The following terms and definitions, abbreviations apply to this document.
3.1 Terms and Definitions
3.1.1 Atomically flat
Are micro-level atomic structure, surface flatness reach the atomic level.
3.2 Acronyms
AFM. atomic force microscope (AtomicForceMicroscope)
DNA. deoxyribonucleic acid (DeoxyribonucleicAcid)
TE. Tris - disodium edetate (TRIS-Ethylenediaminetetraaceticacid, disodiumSalt)
APTES. (3- aminopropyl) - triethoxysilane siloxane [(3-aminopropyl) triethoxysilane]
4. Summary
4.1 According to AFM works
During sample observation should be first to be detected substance is placed in a substrate surface.
4.2
As the DNA molecule is very small, it is required to be placed on the atomically flat and there is no contamination of the substrate.
4.3
Standard utilization characteristics of DNA molecules in an aqueous solution with negatively charged surfaces of new mica substrate cleavage may be modified to bring it
Positively charged, so that the DNA interaction firmly attached to the surface of the substrate by electrostatic mica, to be suitable for subsequent detection of AFM […]
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
Similar standards
How to Download GB/T 32262-2015
- 1
Add to cart
Click "Download PDF" on this page and choose "Buy this standard". You can add more standards before checkout.
- 2
Checkout
Enter your email and billing details. Payment is processed securely by Stripe (cards or bank transfer). Prefer bank details? Request a quotation (min. order $300).
- 3
Instant delivery (0–9 sec)
Delivery is automatic: within seconds of payment you'll receive an email with a secure download link. The link stays valid for 72 hours.
- 4
Invoice included
A tax invoice is attached to the confirmation email. Need a custom invoice? Contact us.
Related Standards
GB/T 10003-2026 — Biaxially oriented polypropylene (BOPP) film
GB/T 10004-2026 — Plastic/Plastic laminated films and pouches for packaging
GB/T 10007-2026 — Rigid cellular plastics - Determination of shear properties
Secure payment via Stripe
Payments accepted
GB/T 32262-2015
$165.00