GB/T 29371.2-2012Technical rules for seed producing system of two-line hybrid rice - Part 2: Technical rules for foundation seed production of photo/thermo-sensitive genic male sterile line (English PDF)

2.1 Artificial climate box

2.1.1 The volume of the artificial climate box

Artificial climate box's interior area is not less than 3m

2 and its height is not lower than 2m.

2.1.2

Parameters settings of light, temperature and humidity of the artificial climate box

It shall comply with provisions indicated in Table 1 and Table 2.

2.2 Phytotron

2.2.1 Structure

The usage area of the single phytotron is 12m

2

- 15m

2 , and the height is 4m - 4.5m. The thickness of the wall is 30m - 40m. It is equipped with the thermal insulation material inside the wall. There is transparent thermal insulation layer at the place 2.0m above the ground. Adopt the 8mm tempered glass. Above the insulation layer, install artificial light source. At the southern-wall, set up a ventilating window with the size of 1m X 1.5m, at

0.5m above the ground. Ventilating window shall adopts the double glazing with the thickness of 5mm - 8mm. It needs to set up a good ventilation system in the phytotron.

And it is equipped with the water supply and water storage device.

2.2.2 Scale

The control system adopts automatic control equipment. And the scale of the control chamber shall be set up according to the number of the phytotrons.

2.2.3 Temperature control equipment

Four sets of wall-mounted air conditioning with the refrigeration and heating capacity of

3kw - 4kw are installed inside the phytotron, one set on every wall, dislocation-installed under the glass thermal insulation layer. Temperature control indicators of the temperature control equipment shall be set within (15°C - 30°C)±1°C.

2.2.4 Artificial light source

The indicator of indoor light intensity is (1.0-2.0) X 10

4 lx. The artificial light source shall adopt the 400W high pressure sodium lamp, in combination with the 400W metal halide lamp. The Ratio is 3:1. The quantity of light source lamps is 15-20, with electrical automatic light-adjustment rectifier.

2.3.3 Temperature control

Set 4 water inlets and outlets respectively. Place the mercury thermometers at the sensitive parts of fertility transform temperature of sterile line of inlets and outlets.

2.4 Natural cold water pond

Use the low-temperature water in reservoir or cold underground water, with the temperature of water source of <=20°C. Depth of cold water pond is >=40cm, with an area of cold water pond of 30m

2

- 60m

2

. The flow rate of cool water is >= 6m

3

/h - 12m

3

/h. A mercury thermometer shall be placed at the position of inlet and outlet.

4.1 Preparation of cultivation pot

Select the plastic pot with a body-height of 20cm-25cm and a diameter of 25 cm-30 cm.

The loading amount of mud shall be appropriate that the mud level is 5cm away from the port edge. The ordinary field soil shall be used.

4.2 Nursery for single-plant

Conduct the seed soaking, germination acceleration, and seedling according to conventional method. When it grows to 5-leaves 1-heart, transplant them into the field, the density is 20 cm X 20 cm, transplant more than 1000 plants of single-plant. Cultivate and manage it according to general field production.

4.3 Transplant

When the stem of single-plant selection nursery plants come into the inflorescence differentiation stage II (japonica rice) or stage 1 (indica rice), select those plants with uniform growth and typical properties of the male sterile line. Transplant into the prepared cultivation pots with soil, on a cloudy day or at the evening time. 4 plants in one pot, or directly transplant them into the cold water pond with soil. Ensure adequate water supply and prevent from insect disease.

4.4 Low temperature processing

4.4.1 Processing in phytotron

5.1 Multiplication methods

Multiplication by cold water irrigation, multiplication in autumn, or multiplication in winter.

5.2 Cultivation and management

The seeds of core single-plant collected are divided into single-plant soaking seed, accelerating germination, sowing, and individual-plant planting. Each single-plant is planted in one plant-line. Field cultivation and management can refer to the multiplication technology of photo/thermo-sensitive genic male sterile line.

5.3 Observation and identification

Respectively observe and identity the plant-lines at tillering period, heading and flowering period, and maturation period. At anytime, eliminate the plant-lines with character variation and segregation.

5.4 Harvesting

Mix-harvest the seeds of selected plant-lines, that is the foundation seed.

Annex A (Normative)

Setting modes of temperature and light of the phytotron processing

Table A.1 Setting modes of temperature and light of the phytotron processing

Temperature Illumination

Time

Daily average temperature 23°C

Daily average temperature 22°C

Time

Light intensity

05:01-11:00 24°C 22°C 06:01-08:00 10

4 lx

11:01-17:00 26°C 26°C 08:01-15:00 1.5×10

4 lx

17:01-23:00 23°C 22°C

15:01-19:30

15:01-20:00 a

10

4 lx

23:01-05:00 19°C 18°C

19:31-06:00

20:01-06:00 a

0 a

Illumination time used when processing japonica rice.

Annex B (Normative)

Photo/thermo-sensitive genic male sterile line rice foundation seed production pollen observation under microscope record table

Table B.1 Photo/thermo-sensitive genic male sterile line rice foundation seed production pollen observation under microscope record table

Name of sterile line: __________ Year

Date (MM/DD)

Plants numbers

View

No staining pollen (grains)

Staining pollen (grains)

Total number of pollens (grains)

Remarks

Observation under microscope person:

Recorder: Technical person in-charge:

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