GB/T 28873-2012General guide of environmental scanning electron microscopy for biological effects on topography induced by nanoparticles (English PDF)
纳米颗粒生物形貌效应的环境扫描电子显微镜检测方法通则
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 5, 2012
Implementation date
February 15, 2013
Scope
GB/T 28873-2012 is the English-translated version of 纳米颗粒生物形貌效应的环境扫描电子显微镜检测方法通则.
GB/T 28873-2012 is the Chinese standard "General guide of environmental scanning electron microscopy for biological effects on topography induced by nanoparticles".
Its scope clause reads: This standard specifies the technical specifications and environmental scanning electron microscopy of nanoparticles with a biological sample interactions.
This standard applies to low vacuum mode of application and environmental vacuum scanning electron microscope to study the biological effects of nanoparticles in biological Analysis of the morphology of the sample (range be extended to sub-micron particles and micron). Its clauses include terms and definitions.
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Document preview — GB/T 28873-2012
National Standard of the People's Republic of China
- ICS
- 17.180
- Classification
- A 60
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 3.1 Environmental Scanning Electron Microscope environmentalscanningelectronmicroscope; ESEM
- 3.2 Biological effects of topography
- 3.3 Low vacuum mode
- 3.4 Environmental vacuum mode
- 3.5 Aqueous biological sample
1 Scope
This standard specifies the technical specifications and environmental scanning electron microscopy of nanoparticles with a biological sample interactions.
This standard applies to low vacuum mode of application and environmental vacuum scanning electron microscope to study the biological effects of nanoparticles in biological
Analysis of the morphology of the sample (range be extended to sub-micron particles and micron).
2 Normative references
The following documents for the application of this document is essential. For dated references, only the dated version suitable for use herein
Member. For undated references, the latest edition (including any amendments) applies to this document.
GB/T 19619-2004 nanomaterials term
GB/T 21636-2008 Micro cytometry analysis electron probe microanalysis (EPMA) term
GB/T 23414-2009 micro cytometry analysis Scanning Electron Microscopy term
3 Terms and Definitions
The following terms and definitions apply to this document.
3.1 Environmental Scanning Electron Microscope environmentalscanningelectronmicroscope; ESEM
In its work, the pressure in the sample chamber between 1Pa ~ 5000Pa change. Therefore, it can not directly detect the secondary electron emission
Son, the image is enlarged by detecting electron gas, environmental scanning electron detector to detect ions, the photoelectric effect or other detection signal (such as
Backscattered electrons or sink current) is obtained.
3.2 Biological effects of topography
After the nanoparticles, biological sample morphology changes.
3.3 Low vacuum mode
In the high pressure vacuum tube, and the sample chamber at 10Pa ~ 130Pa, the surface of the sample for testing.
3.4 Environmental vacuum mode
In the high pressure vacuum tube, and the sample chamber is in the range 10Pa ~ 4000Pa, the detection surface of the sample.
3.5 Aqueous biological sample
Surface without the presence of excess water, internal water, easily deformed fresh samples.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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