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GB/T 28090-2025Detection and identification of Euphrosyne xanthiifolia (Nutt.) A. Gray (English PDF)

假苍耳检疫鉴定方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

May 30, 2025

Implementation date

December 1, 2025

Scope

GB/T 28090-2025 is the English-translated version of 假苍耳检疫鉴定方法.

GB/T 28090-2025 is the Chinese national standard covering the quarantine identification of false cocklebur, a weed of the Asteraceae intercepted at the border — the classification information and the morphology of the involucre, the paleae and the achene, the field sampling, the apparatus and reagents, the preparation and observation of the sample, the ITS sequencing and the real-time fluorescence PCR, the separation from the similar genera an inspector is most likely to confuse it with, and the result determination. The appendix on the similar genera is the part that does the work, because a single achene is often all an inspector has to decide on. Issued on 30 May 2025, it has been in force since 1 December 2025, replacing GB/T 28090-2011.

Document preview — GB/T 28090-2025

National Standard of the People's Republic of China

ICS
65.020.20
Classification
B 16
Replacing
GB/T 28090-2011

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms, definitions and abbreviations
  • 3.1 Terms and Definitions
  • 3.1.1 Bract phylary
  • 3.1.2 Involucre
  • 3.1.3 Palea
  • 3.1.4 Achene
  • 3.2 Abbreviations
  • 4 Classification information of Pseudo-Xanthium

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for standardization work Part 1: Structure and drafting rules for standardization documents" Drafting.

This document replaces GB/T 28090-2011 "Identification Method for Quarantine of Xanthium sibiricum". Compared with GB/T 28090-2011, in addition to the structural adjustment In addition to editorial changes, the main technical changes are as follows.

— Changed "Scope" (see Chapter 1, Chapter 1 of the 2011 edition);

— The term "capitate" has been deleted, the definitions of the terms "involucre" and "achene" have been changed, and the terms "involucre" and "involucre" have been added (see Chapter 3, Chapter 2 of the 2011 edition);

— Changed the "Classification information of Xanthium sibiricum" (see Chapter 4, Chapter 3 of the 2011 edition);

— Changed "Apparatus and Reagents" (see Chapter 6, Chapter 5 of the 2011 edition);

— Deleted "Laboratory Testing and Identification" (see Chapter 6 of the 2011 edition);

— Added "Field Sampling" and "Observation Methods and Sample Preparation" (see Chapters 7 and 8);

— Changed "Identification Method" (see Chapter 9, 6.3 of the 2011 edition);

— Changed "Result Determination" (see Chapter 10, Chapter 8 of the 2011 edition);

— Changed "Sample Storage and Handling" (see Chapter 11, Chapter 9 of the 2011 edition);

— Added morphological characteristics of Pseudocalli and similar genera (see Appendix B);

— The morphological characteristics of Xanthium sibiricum have been changed (see Appendix C, Appendix B and Appendix C of the 2011 edition);

— Added ITS sequence amplification and sequencing information and real-time fluorescence PCR detection of Pseudo-Xanthium sibiricum (see Appendix D and Appendix E).

Please note that some of the contents of this document may involve patents. The issuing organization of this document does not assume the responsibility for identifying patents.

This document is under the jurisdiction of the National Technical Committee on Plant Quarantine Standardization (SAC/TC271).

This document was drafted by: China Institute of Inspection and Quarantine, Ningxia Hui Autonomous Region Agricultural Technology Extension Center, China Customs Science and Technology Technical Research Center, Erlian Customs Technical Center, and Alashankou Customs Technical Center.

The main drafters of this document are: Xu Han, Du Wei, Zhao Xiaoli, Zhai Junfeng, Yuan Shuzhen, Li Lan, Liu Fang, Tian Qian, Liu Mengdi, Zhang Ting and Li Zongze.

The previous versions of this document and the documents it replaces are as follows.

— First published in 2011 as GB/T 28090-2011;

— This is the first revision.

Quarantine and identification method of Pseudo-Xanthium sibiricum

1 Scope

method.

This document is applicable to the quarantine and identification of Pseudo-Xanthium sibiricum.

2 Normative references

This document has no normative references.

3 Terms, definitions and abbreviations

3.1 Terms and Definitions

The following terms and definitions apply to this document.

3.1.1 Bract phylary

Bracts arising from the outermost or lower part of the inflorescence.

3.1.2 Involucre

It is composed of multiple bracts, forming one or more whorls of structures that wrap around the inflorescence.

3.1.3 Palea

The scale-like bracts on the inflorescence receptacle of the Asteraceae family. Note. The plural is paleae.

3.1.4 Achene

A small, dry fruit with a hard, indehiscent skin containing one seed.

Note. The apex is usually covered with pappus or other appendages and the base is usually connected to the fruiting pedicel.

3.2 Abbreviations

The following abbreviations apply to this document. Ct. cycle threshold ITS. internal transcribed spacer 1 and internal transcribed spacer 2 of ribosomal DNA, and 5.8S ribosomal gene (internaltranscribed

4 Classification information of Pseudo-Xanthium

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 24 pages — is available in the English PDF.

Editions of GB/T 28090

EditionTitleRevisionStatus
GB/T 28090-2025Detection and identification of Euphrosyne xanthiifoliacurrent editionCurrent
GB/T 28090-2011Detection and identification of Iva xanthifolia Nuttprevious editionIn force

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