GB/T 28073-2011Detection and identification of Arabis mosaic virus (English PDF)

1 Scope

This standard specifies the method for detection and identification of Arabis mosaic virus serology and molecular biology.

This standard applies to plant seeds, the detection and identification of scales bulbs, seedlings and tissue culture and other plants and their products SOUTH Arabis mosaic virus.

2 Arabis mosaic virus Basic Information

Chinese name. Arabis mosaic virus.

English name. arabismosaicvirus.

Synonyms. arabismosaicnepovirus; hopnettleheadvirus (hop nettle disease virus); rhubarbmosaicvirus (large

Yellow mosaic virus); raspberryyelowdwarfvirus (Rubus yellow dwarf virus); ashringandlinepatternviurs (ash tree

Ring-like virus); rhabarber-mosaik-virus (rhubarb mosaic virus); forsythiayelownetvirus (forsythia yellow mesh virus);

jasmineyelowblotchvirus (Jasmine macular virus).

English abbreviation. ArMV.

Genus cowpea mosaic virus family Comoviridae, nematodes pass polyhedron virus genus Nepovirus.

For additional information, see Appendix A. Arabis mosaic virus

3 PRINCIPLE OF THE METHOD

The use of double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on antigen-antibody reaction, reverse transcription and in vitro DNA amplification technology

Surgery reverse transcription polymerase chain reaction (RT-PCR) detection and identification.

4 Main equipment

The standard detection methods identified mainly with the following equipment.

Trace juicer, microplate reader, washer, microbalance (sense of volume. 0.001g), PCR, fluorescence PCR, electrophoresis, horizontal electrophoresis

Groove, gel imager, desktop high-speed refrigerated centrifuge, water bath, pH meter, and a variety of ranges adjustable pipette (1000µL, 200µL,

100µL, 20µL, 10µL, 2µL).

5 Detection and Identification