GB/T 26938-2023Code of practice for in vivo embryo production and transfer in cattle (English PDF)

Foreword

This document is in accordance with the provisions of GB/T 1.1-2020 "Guidelines for Standardization Work Part 1: Structure and Drafting Rules for Standardization Documents" drafting.

This document replaces GB/T 26938-2011 "Technical Regulations for Bovine Embryo Production". Compared with GB/T 26938-2011, except for structural adjustments In addition to editorial changes, the main technical changes are as follows:

--- Added the term "receptor" (see 3.1.2);

--- Added abbreviations (see 3.2);

--- Increased technical process (see Chapter 4);

--- Changed the selection of donors (see 5.1, 4.1 of the 2011 edition);

--- Changed the feeding management of the donor (see 5.2, 4.2 of the 2011 edition);

---Changed the estrus synchronous treatment of the donor (see 6.1.1, 5.1.1 of the 2011 edition);

---Changed the superovulation method (see 6.1.2, 5.1.2 of the 2011 edition);

--- Changed the donor estrus identification (see 6.1.3, 5.1.4 of the 2011 edition);

--- Change breeding to artificial insemination (see 6.2, 5.2 of the 2011 edition);

--- Added site conditions (see 7.1);

---Changed non-surgical embryo collection (see 7.2, 6.1 of the 2011 edition);

--- Changed the selection of embryos and embryo quality identification (see 7.3, 7.4, 6.2 of the 2011 edition);

--- Increased embryo washing (see 7.5);

--- Changed the freezing and preservation of embryos, and added thawing of embryos (see Chapter 8, Chapter 7 of the 2011 edition);

--- Added embryo transfer and pregnancy test (see Chapter 9);

--- Increased verification method (see Chapter 10);

---Changed the main instruments required for non-surgical embryo collection (see A.4 in Appendix A, A.3 in Appendix A of the 2011 edition).

1 Scope

This document establishes the technical process of bovine embryo production and transplantation, specifies the selection and feeding management of donors, the superovulation and Artificial insemination, non-surgical embryo collection and embryo quality identification, embryo cryopreservation and thawing, embryo transfer and pregnancy inspection technical operations As required, the verification method is described.

This document is applicable to the technical operation of ordinary bovine embryo production and transplantation.

2 Normative references

The contents of the following documents constitute the essential provisions of this document through normative references in the text. Among them, dated references For documents, only the version corresponding to the date is applicable to this document; for undated reference documents, the latest version (including all amendments) is applicable to this document.

GB 4143 Bovine frozen semen

GB/T 5458 Liquid nitrogen biological container

GB/T 25881 Bovine Embryo

GB/T 31582 Bovine sex-controlled frozen semen

NY/T 1335 Cattle Artificial Insemination Technical Regulations

3 Terms, Definitions and Abbreviations

4 Technical process

In vivo embryo production and transplantation techniques in cattle include donor selection and feeding management, donor superovulation and artificial insemination, non-surgical embryo