GB/T 24253-2009Textiles - Evaluation for anti-mites activity (English PDF)

3.1 Mites

A kind of tiny animal that belongs to Arthropoda, Arachnida, Acari, with the body of small spherical or elongated shape. The basic structure is divided into two parts. jaw and body. Mites at adult and nymph stage have four pairs of legs, the larvae have three pairs of legs.

3.2 Anti-mites activity

Activity of products to repellent mites or inhibit the growth and reproduction of mites.

3.3 Control sample

Materials that are used to validate the growth conditions of test mites; it shall use the same materials with the sample but without anti-mites finishing. If necessary, it can also use unprocessed 100% cotton fabric, after being high-temperature cooked and washed with distilled water, as the control sample.

6.1 Dissecting microscope or stereo microscope

6.9 Oven

7.1 Mite species

The test mites in this Standard adopt male and female adult mites or nymph mites of dust mites (Dermatophagoides farinae Hughhes).

7.2 Marks

Each kind of test mites shall be marked with the following information.

8.1 Sample size

Fabric. SELECT representative specimens from each sample, CUT them into a circular with a diameter of 58 mm as a sample.

8.2 Sample pretreatment

PLACE the sample at dry heat condition of 65°C ± 5°C for 10 min.

8.3 Sample washing

If samples' anti-mites and washability activity is to be tested, WASH the 3 samples on 8.1 according to the test condition A1M in GB/T 12490-2007; one cycle is equivalent to 5 times of washing (the specific operation of one cycle.

9.1 Repellent method

9.1.1 PUT a piece of sponge with a thickness of 10 mm and a side length of about 200 mm INTO a covered container, ADD a suitable amount of saturated brine (6.10) (the height of water just immerses the sponge).

9.2 Inhibition method

9.2.1 PUT a piece of sponge with a thickness of 10 mm and a side length of about 200 mm INTO a covered container, ADD a suitable amount of saturated brine (6.10) (the height of water just immerses the sponge).

9.2.2 PLACE respectively 3 samples and 3 control samples IN 6 petri dishes, LAY the samples ON the bottom of the petri dishes uniformly, smoothly and tightly, and PUT 0.05 g of mites feed uniformly ON the samples.

9.2.5 COVER the container, PLACE the container IN a constant-temperature-and-humidity incubator at a temperature of (25 ± 2)°C and a relative humidity of (75 ± 5)%.

9.2.6 After developed for 7 d, 14 d, 28 d or 42 d as needed, use a dissecting microscope to observe and record the number of living adult mites and nymph mites in the petri dishes.

9.2.3 PUT 150 living mites IN 6 petri dishes […]