GB/T 23814-2009Protocol of the polymerase chain reaction for detecting kidney beans components in lotus foods (English PDF)
莲蓉制品中芸豆成分定性PCR检测方法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
May 27, 2009
Implementation date
September 1, 2009
Scope
GB/T 23814-2009 is the English-translated version of 莲蓉制品中芸豆成分定性PCR检测方法.
GB/T 23814-2009 is the Chinese standard "Protocol of the polymerase chain reaction for detecting kidney beans components in lotus foods". Its scope clause reads: This standard specifies the qualitative PCR method to detect kidney beans ingredient lotus seed paste products.
This standard applies to semi-finished and finished products by the lotus seed paste ingredients in beans qualitative detection. The standard detection limit of 0.5ng beans DNA.
Its clauses include terms and definitions; anti-pollution measures; sampling and sample preparation; determination.
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Document preview — GB/T 23814-2009
National Standard of the People's Republic of China
- Classification
- X 04
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 3 Terms and Definitions
- 4 anti-pollution measures
- 5 Sampling and sample preparation
- 6 Determination
1 Scope
This standard specifies the qualitative PCR method to detect kidney beans ingredient lotus seed paste products.
This standard applies to semi-finished and finished products by the lotus seed paste ingredients in beans qualitative detection.
The standard detection limit of 0.5ng beans DNA.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
GB/T 19495.1 GMO testing - General requirements and definitions
GB/T 19495.2 technical requirements for GMO testing laboratories
GB/T 19495.7 GMO detection methods of sampling and sample preparation
3 Terms and Definitions
Terms and definitions GB/T 19495.1 established apply to this standard.
4 anti-pollution measures
Measures to prevent cross-contamination detection process carried out according to GB/T 19495.2 provisions.
5 Sampling and sample preparation
Execution method according to GB/T 19495.7 requirements.
6 Determination
6.1 Principle
The samples were extracted DNA, gene sequence specific for kidney primers were designed by the PCR technique, the gene-specific amplification beans
DNA fragments, according to the results of PCR amplification to determine whether the sample contains beans ingredients.
6.2 Reagents and materials
Unless otherwise specified, the reagents used were of analytical grade or biochemical reagents, water as a water according to GB/T 6682 regulations.
6.2.1 primer
primer and product information detecting endogenous gene lotus seed paste and kidney beans specific genes in Table 1.
Table 1 Primer Information lotus seed products, the desired foreign gene
Detection of gene primer sequences
PCR product size / bp
Gene nature Scope
NNEA0732
Positive. 5'-GTAGAATCGGCACTGGAGGTAG-3 '
Anti-. 5'-CATGGGCTAACCAACTAGACAGC-3 '
142 lotus seed lotus seed specific gene products
AB070627
Positive. 5'-CAGACATTTCATATCCAGGGAGG-3 '
Anti-. 5'-TGAATTGTACGGTGAAGGATGG-3 '
194 kidney beans, lotus seed paste specific gene products
GB/T 23504-2009 | GB/T 22507-2008 | GB/T 22509-2008 | GB/T 23817-2009 | GB/T 23816-2009 | GB/T 23815-2009 | […]
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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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