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GB/T 23814-2009Protocol of the polymerase chain reaction for detecting kidney beans components in lotus foods (English PDF)

莲蓉制品中芸豆成分定性PCR检测方法

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Issued by

SAMR; SAC

Level / Type

National · Recommended

Issue date

May 27, 2009

Implementation date

September 1, 2009

Scope

GB/T 23814-2009 is the English-translated version of 莲蓉制品中芸豆成分定性PCR检测方法.

GB/T 23814-2009 is the Chinese standard "Protocol of the polymerase chain reaction for detecting kidney beans components in lotus foods". Its scope clause reads: This standard specifies the qualitative PCR method to detect kidney beans ingredient lotus seed paste products.

This standard applies to semi-finished and finished products by the lotus seed paste ingredients in beans qualitative detection. The standard detection limit of 0.5ng beans DNA.

Its clauses include terms and definitions; anti-pollution measures; sampling and sample preparation; determination.

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Document preview — GB/T 23814-2009

National Standard of the People's Republic of China

Classification
X 04

Issued by: State Administration for Market Regulation; Standardization Administration of the PRC

Contents

  • 1 Scope
  • 2 Normative references
  • 3 Terms and Definitions
  • 4 anti-pollution measures
  • 5 Sampling and sample preparation
  • 6 Determination

1 Scope

This standard specifies the qualitative PCR method to detect kidney beans ingredient lotus seed paste products.

This standard applies to semi-finished and finished products by the lotus seed paste ingredients in beans qualitative detection.

The standard detection limit of 0.5ng beans DNA.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

GB/T 19495.1 GMO testing - General requirements and definitions

GB/T 19495.2 technical requirements for GMO testing laboratories

GB/T 19495.7 GMO detection methods of sampling and sample preparation

3 Terms and Definitions

Terms and definitions GB/T 19495.1 established apply to this standard.

4 anti-pollution measures

Measures to prevent cross-contamination detection process carried out according to GB/T 19495.2 provisions.

5 Sampling and sample preparation

Execution method according to GB/T 19495.7 requirements.

6 Determination

6.1 Principle

The samples were extracted DNA, gene sequence specific for kidney primers were designed by the PCR technique, the gene-specific amplification beans

DNA fragments, according to the results of PCR amplification to determine whether the sample contains beans ingredients.

6.2 Reagents and materials

Unless otherwise specified, the reagents used were of analytical grade or biochemical reagents, water as a water according to GB/T 6682 regulations.

6.2.1 primer

primer and product information detecting endogenous gene lotus seed paste and kidney beans specific genes in Table 1.

Table 1 Primer Information lotus seed products, the desired foreign gene

Detection of gene primer sequences

PCR product size / bp

Gene nature Scope

NNEA0732

Positive. 5'-GTAGAATCGGCACTGGAGGTAG-3 '

Anti-. 5'-CATGGGCTAACCAACTAGACAGC-3 '

142 lotus seed lotus seed specific gene products

AB070627

Positive. 5'-CAGACATTTCATATCCAGGGAGG-3 '

Anti-. 5'-TGAATTGTACGGTGAAGGATGG-3 '

194 kidney beans, lotus seed paste specific gene products

GB/T 23504-2009 | GB/T 22507-2008 | GB/T 22509-2008 | GB/T 23817-2009 | GB/T 23816-2009 | GB/T 23815-2009 | […]

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This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.

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