GB/T 23502-2009Determination of ochratoxin A in food -- High performance liquid chromatographic method with immunoaffinity column clean-up (English PDF)

Foreword

Appendix A of this standard is an informative annex.

This standard by the National Food Industry Standardization Technical Committee (SAC/TC64) proposed.

This standard by the National Standardization Technical Committee on Food Universal Detection Technology Food Industry Technical Committee (SAC/TC64/SC8) Centralized.

This standard was drafted. Qingdao City Product Quality Supervision and Inspection, People's Republic of China Shandong Exit Inspection and Quarantine, the inspection Wellcome Technology Co., Ltd., Tsingtao Brewery.

1 Scope

This standard specifies the food content of ochratoxin A immunoaffinity chromatography and HPLC purification method.

This standard applies to the determination of food products, wine, soy sauce, vinegar, soy sauce and butter products of ochratoxin A in food and agriculture.

The detection limit of this standard. food and food products, the detection limit was 1.0µg/kg, alcohol detection limit of 0.1µg/kg, soy sauce, Vinegar, soy sauce and sauce products, detection limit is 0.5µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

3 Method summary

Extract sample was extracted with ochratoxin A, after immunoaffinity column purified by high performance liquid chromatography with fluorescence detector, external Standard method.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water line with GB/T 6682 regulations.

4.1 Methanol. chromatographically pure.

4.2 acetonitrile. chromatography.

4.3 glacial acetic acid. Chromatographic pure.

4.4 1 extract. methanol + water (80 + 20).

4.5 extract 2. Weigh 150g of sodium chloride, 20g of sodium bicarbonate dissolved in about 950mL of water, add water to 1L.

4.6 Rinse solution. Weigh 25g sodium chloride, 5g of sodium bicarbonate dissolved in about 950mL of water, add water to 1L.

4.7 Mycotoxins washing buffer. Weigh 25.0g NaCl, 5.0g of sodium bicarbonate dissolved in water was added 0.1mL Tween-20, washed with water Diluted to 1L.

4.8 ochratoxin A standard. Purity >=98%.

4.9 Ochratoxin A stock standard solution. Weigh accurately a certain amount of ochratoxin A standard, dissolved in methanol and acetonitrile + (1 + 1), Dubbed 0.1mg/mL standard stock solution stored at -20 °C, can be used for 3 months.

4.10 ochratoxin A standard working solution. Depending on needs, Imbibe ochratoxin A certain amount of stock solution, dilute with mobile phase Release, dubbed respectively equivalent to 1ng/mL, 5ng/mL, 10ng/mL, 20ng/mL, working standard solution 50ng/mL of, 4 °C preservation, can Use 7d.

4.11 ochratoxin A immunoaffinity column.

4.12 glass fiber filter. diameter 11cm, pore size 1.5µm, no fluorescent properties.

5 Apparatus

5.1 Balance. a sense of the amount of 0.001g.