GB/T 23224-2008Identification of cultivar resistance to tobacco disease (English PDF)

3.1

Pathogen due to mutations, hybridization, adaptive variation, different heterogeneity of spore cytoplasm and other species cause physiological changes, resulting in pathogenicity difference.

3.2

Artificial breeding or collected pathogens, inoculated by a certain amount, and create conditions for the incidence, disease-resistant varieties is determined according to disease severity vaccination

Of strength.

3.3

In order to test the reliability of the test, in the identification of additional varieties of disease-resistant varieties and susceptible varieties.

4.1 blackleg

4.1.1 Identification of field disease nursery

4.1.1.1

Identification of

Select facilitate irrigation and drainage, fertility levels moderate in recent years the incidence of heavy tobacco field for identification garden, nurseries should be clear pathogens physiological categories. each

Varieties planted 1 lines of 20 or more, at least 3 repeats, random order, to repeat each set of resistant varieties "Renewing III", in resistant varieties

"Venus 6007" and susceptible cultivars "Little Gold 1025" control the behavior of each one. Separate anti locally grown, the sense of variety as auxiliary controls.

Senate Kam tobacco varieties in the growth period should not be used fungicides, insecticides according to kind and degree of occurrence of the pest in the garden depending on the disease.

Artificial inoculation, the inoculum using local advantages physiological races or strains selected according to the test required. After tobacco survived moved to Daejeon (10d ~ 20d) were inoculated with bacteria Valley, per plant inoculation 4g. If the dry weather after vaccination should be irrigation, soil moisture so that the field is saturated status.