GB/T 22994-2008Determination of levamisole residues in milk and milk powder - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the milk and milk powder levamisole residues in liquid chromatography - tandem mass spectrometry.

This standard applies to liquid milk (including raw milk, whole milk, skim milk) and milk (including plain milk, skim milk powder and infant formula

Milk) levamisole (levamisole) Determination of the residual.

This standard milk Levamisole detection limit of 0.4µg/kg; milk powder levamisole detection limit of 3.2µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-

2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Extracting a sample in an alkaline environment Levamisole residue with ethyl acetate and dilute hydrochloric acid. The extract was converted to the hydrochloride levamisole

Levamisole hydrochloride and Anti-mentioned layer, strong cation exchange solid phase extraction (SPE), liquid chromatography - tandem mass spectrometer, external standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade.