GB/T 22990-2008Determination of oxytetracycline, tetracycline, chlortetracycline, doxycycline residues in milk and milk powder - HPLC-UV method (English PDF)

1 Scope

This standard specifies the milk and milk powder oxytetracycline, tetracycline, chlortetracycline, and doxycycline residues LC - UV detection Determination method.

This standard applies to milk and oxytetracycline, tetracycline, chlortetracycline, doxycycline residues in milk powder Determination.

The standard method detection limit. milk oxytetracycline, tetracycline 5µg/kg, chlortetracycline, doxycycline is 10µg/kg. Milk Turkey

Neomycin, tetracycline 25µg/kg, chlortetracycline, doxycycline is 50µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-

2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

With 0.1mol/LNa2EDTA-Mclvaine sample extraction buffer solution Tetracycline antibiotic residues, OasisHLB or equivalent

Solid phase extraction column and a carboxylic acid type cation exchange column purification, liquid chromatograph, external standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.