GB/T 22984-2008Determination of the residues of metabolites of carbadox and olaquindox in milk and milk powder - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the milk and milk powder carbadox metabolite quinoline

2-carboxylic acid (quinoxaline-2-carboxylicacid) and olaquindox

Metabolite 3-methyl-2-carboxylic acid (3-methylquinoxaline-2-carboxylicacid) residual amount of liquid chromatography - tandem mass spectrometry method.

This standard applies to milk and milk powder carbadox metabolite quinoline

2-carboxylic acid (quinoxaline-2-carboxylicacid) and olaquindox

Metabolite 3-methyl-2-carboxylic acid (3-methylquinoxaline-2-carboxylicacid) Determination of the residual.

The standard method detection limit. quinoline

2-carboxylic acid and 3-methyl-quinoline-2-carboxylic acid detection limit milk 0.5µg/kg, milk

Of 4.0µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

The sample was digested with formic acid solution, so that milk and milk powder naturally occurring enzyme inactivation and then hydrolysis, hydrochloric acid, centrifuged

After filtration, the solid-phase extraction (SPE), liquid chromatography - tandem mass spectrometer, internal standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.