GB/T 22977-2008Determination of phenylbutazone residues in bovine milk and milk powder - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the milk and milk powder mediator phenylbutazone residues in liquid chromatography - tandem mass spectrometry.

This standard applies to the determination of milk and milk powder mediator phenylbutazone residues.

The standard method detection limit. milk 5.0µg/kg, milk 40.0µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Milk and milk powder mediator phenylbutazone residues with aqueous ammonia to maintain an alkaline condition, remove the fat with alcohol, ether and petroleum ether, and then in the acidic conditions

Under pieces with tetrahydrofuran - Extraction Bute residual hexane mixed solution was concentrated volume for liquid chromatography - tandem mass spectrometer. External standard method

Quantified.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade.