GB/T 22976-2008Determination of alpha-trenbolone, beta-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder - LC-MS-MS method (English PDF)
牛奶和奶粉中α-群勃龙、β-群勃龙、19-乙烯去甲睾酮和epi-19-乙烯去甲睾酮残留量的测定 液相色谱-串联质谱法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
December 31, 2008
Implementation date
May 1, 2009
Scope
GB/T 22976-2008 is the English-translated version of 牛奶和奶粉中α-群勃龙、β-群勃龙、19-乙烯去甲睾酮和epi-19-乙烯去甲睾酮残留量的测定 液相色谱-串联质谱法.
GB/T 22976-2008 is the Chinese standard "Determination of alpha-trenbolone, beta-trenbolone, nortestosterone and epi-nortestosterone residues in milk and milk powder - LC-MS-MS method".
Its scope clause reads: This standard specifies the milk and milk powder alpha- trenbolone, beta- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone Residues High performance liquid chromatography - tandem mass spectrometry.
This standard applies to milk and milk powder alpha- trenbolone, beta- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone Residues Determination and confirmation.
The detection limit of the standard methods. milk alpha- trenbolone, beta- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone as 1µg/kg, milk powder alpha- trenbolone, beta- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone was 5µg/kg. Its clauses include reagents and materials.
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Document preview — GB/T 22976-2008
National Standard of the People's Republic of China
- Classification
- X 04
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 4 Reagents and materials
- 4.6 anhydrous sodium sulfate, AR
1 Scope
This standard specifies the milk and milk powder alpha- trenbolone, beta- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone Residues
High performance liquid chromatography - tandem mass spectrometry.
This standard applies to milk and milk powder alpha- trenbolone, beta- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone Residues
Determination and confirmation.
The detection limit of the standard methods. milk alpha- trenbolone, beta- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone as
1µg/kg, milk powder alpha- trenbolone, beta- trenbolone 19-nortestosterone ethylene and ethylene-epi-19- nortestosterone was 5µg/kg.
2 Normative references
The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-
2004, ISO 5725-1.1994, IDT)
GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat
Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
Principle 3
Sample at pH5.0 enzyme hydrolysis conditions, acetonitrile - ethyl acetate extraction, gel chromatography purification by high performance liquid chromatography - tandem mass spectrometry measurement
Fixed, external standard.
4 Reagents and materials
Unless otherwise stated, all reagents HPLC grade water to a water GB/T 6682 regulations.
4.1
acetonitrile.
4.2
ethyl acetate.
4.3
cyclohexane.
4.4
methanol.
4.5
acetic acid.
4.6 anhydrous sodium sulfate, AR
After burning 650 °C 4h, placed in the dryer set aside.
4.7
sodium acetate, analytical grade.
pH = 5.0.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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