GB/T 22967-2008Determination of beta-estradiol residues in milk and milk powder - Gas chromatography-negative-ion chemical ionization mass spectrometry method (English PDF)

1 Scope

This standard specifies the milk and milk powder beta- estradiol residues in gas chromatography - negative chemical ionization mass spectrometry.

This standard applies to the determination of milk and milk powder beta- estradiol residues.

The standard method detection limit. milk 0.25µg/kg, milk 2µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Milk and milk powder estradiol after enzymatic hydrolysis with acetonitrile as the extraction and extraction agent, protein precipitant, solid-phase extraction column purification,

After acylation derivatized by gas chromatography - negative chemical ionization mass spectrometry and selected ion monitoring mode measurement, internal standard.

4 Reagents and materials

Unless otherwise specified, reagents were of analytical grade water as a water GB/T 6682 regulations.