GB/T 22964-2008Determination of lincomycin, oleandomycin, erythromycin, tilmicosin, tylosin, spiramycin, kitasamycin and josamycin residues in fugu and eel - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the puffer fish and eel lincomycin, oleandomycin, erythromycin, tilmicosin, tylosin, spiramycin, guitar mildew

Su and josamycin residues liquid chromatography - tandem mass spectrometry.

This standard applies to puffer fish and eel lincomycin, oleandomycin, erythromycin, tilmicosin, tylosin, spiramycin, guitar mildew

Su and josamycin residues determination.

The standard method detection limit. puffer fish lincomycin, oleandomycin, erythromycin, tilmicosin, tylosin, spiramycin, Kyrgyzstan

He neomycin and josamycin were 2.0µg/kg. Eel lincomycin, erythromycin, tylosin, neomycin guitar were 2.0µg/kg,

Spiramycin, oleandomycin, josamycin, tilmicosin were 5.0µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Puffer fish and eels in lincomycin, oleandomycin, erythromycin, tilmicosin, tylosin, spiramycin, josamycin guitar and neomycin

The residue was extracted with Tris buffer solution by OasisHLB SPE column 1) purification, methanol eluate was concentrated to volume liquid

Tandem mass spectrometry, internal standard - chromatography.

1) OasisHLB SPE waters is the company's product trade name, this information is given for the convenience of users of this standard, does not mean

Endorsement of the product. If other equivalent product has the same effect, you can use these equivalent products.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade.