GB/T 22963-2008Determination of zearalanol, zearalanone, diethylstilbestrol, hexestrol and dienoestrol residues in fugu, eels and baked eel - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the puffer fish, eel and eel in zeranol (zearalanol), Zearalanone (zearalanone), diethylstilbestrol (Diethylstilbestrol), hexoestrol (hexestrol), double diethylstilbestrol (dienoestrol) residual amount of liquid chromatography - tandem mass spectrometry.

This standard is used in puffer fish, eel and eel in zeranol, Zearalanone, diethylstilbestrol, hexoestrol, double the amount of residual diethylstilbestrol

Determination.

The standard method detection limit. zeranol, Zearalanone, hexane estrone, diethylstilbestrol and diethylstilbestrol double to 1.0µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

With tert-butyl methyl ether and acetate buffer plus enzymatic agents were extracted from the sample remaining zeranol, Zearalanone, hexene female

Phenol, hexane estrone, diethylstilbestrol double by silica gel column purification using liquid chromatography - tandem mass spectrometer, internal standard.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade.