GB/T 22959-2008Determination of chloramphenicol, thiamphenicol and florfenicol residues in fugu, eel and baked eel - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the puffer fish, eels and eel chloramphenicol (chloramphenicol), thiamphenicol (thiamphenicol) and florfenicol

Test (florfenicol) and its metabolite florfenicol amine (florfenicolamine) residual amount of liquid chromatography - tandem mass spectrometry.

This standard applies to fish, eels and eel chloramphenicol, thiamphenicol, florfenicol and florfenicol measured puffer amine residues.

The standard method detection limit. puffer fish, eels and eel chloramphenicol was 0.1µg/kg, thiamphenicol, florfenicol and florfenicol

Amine is 1.0µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-

2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Samples of chloramphenicol, thiamphenicol, florfenicol and florfenicol amine under basic conditions, extracted with ethyl acetate, and the extract rotary evaporation

Play dryness and the residue was dissolved in water, liquid-liquid distribution defatted with hexane. Liquid chromatography - tandem mass spectrometer, chloramphenicol, thiamphenicol and fluorobenzene

Nikau quantitative standard method of peak area, florfenicol quantitative standard method of peak area to the amine.

4 Reagents

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.