GB/T 22950-2008Determination of 12beta-agonists residues in fugu, eel and baked eel - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the puffer fish, eels and eel of 12 beta- agonists residues in liquid chromatography - tandem mass spectrometry.

This standard applies to puffer fish, eels and eel of 12 multi-residue determination of beta- stimulants. 12 kinds of stimulant medications include bromine cloth beta-

Castro (brombuterol), Zeeman Castro (cimaterol), clenbuterol (clenbuterol), clenbuterol Pointe (clenpenterol), hydroxymethyl ammonia

Clenbuterol (hydroxymethylclenbuterol), phenoxy propofol amine (isoxsuprine), Maputo Castro (mabuterol), ractopamine (Ractopamin), ritodrine (ritodrine), albuterol (salbutamol), terbutaline (terbutaline) and tulobuterol (Tulobuterol).

This standard ractopamine, salbutamol, Zeeman Castro, clenbuterol and clenbuterol Pointe method detection limit of 0.1µg/kg, bromine cloth

Castro, tulobuterol, Maputo Castro, terbutaline, ritodrine, phenoxy propofol amine and ammonia hydroxymethyl clenbuterol detection limit of the method

0.5µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Dilute acid hydrolysis samples after protein precipitation with perchloric acid, the residue of beta- agonists with ethyl acetate and isopropyl alcohol mixed solvent, mixed

Cation exchange adsorption inverted solid phase extraction column purification, liquid chromatography - tandem mass spectrometry assay, internal standard method.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade water as a water GB/T 6682 regulations.