GB/T 22948-2008Determination of trimethoprim residues in royal jelly - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the royal jelly Three trimethoprim residues in liquid chromatography - tandem mass spectrometry.

This standard applies to the determination of three royal jelly trimethoprim residues.

The standard method detection limit of 2.0µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Three residues in royal jelly trimethoprim deionized water extraction, TCA protein, centrifuged, the supernatant by Oasis

MCX ion exchange column 1) or equivalent solid-phase extraction (SPE) with ammonia - methanol eluted and evaporated and the residue with acetonitrile and 0.1% formic acid

Dissolved. Sample solution for chromatography - tandem mass spectrometer, external standard.

1) OasisMCX ion exchange columns are Waters company's product trade name, this information is given for the convenience of users of this standard, not the table

Endorsement of the product shown. If other equivalent product has the same effect, you can use these equivalent products.

4 Reagents and materials