GB/T 22946-2008Determination of lincomycin, erythromycin, tilmicosin, tylosin, spiramycin, clindamycin, kitasamycinand josamycin residues in royal jelly and lyophilized royal jelly powder - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the royal jelly and royal jelly lyophilized lincomycin, erythromycin, tilmicosin, tylosin, spiramycin, clindamycin

Su, guitar neomycin and josamycin residues liquid chromatography - tandem mass spectrometry.

This standard applies to royal jelly and royal jelly powder lincomycin, erythromycin, tilmicosin, tylosin, spiramycin, clindamycin

Su, guitar neomycin and josamycin residues determination.

The standard method detection limit. Royal Jelly lincomycin, erythromycin, tilmicosin, tylosin, spiramycin, clindamycin, Kyrgyzstan

He adriamycin, josamycin were 2.0µg/kg; royal jelly freeze-dried powder of lincomycin, erythromycin, tylosin, clindamycin, josamycin

Were 2.0µg/kg, tilmicosin, spiramycin, guitar ADM were 5.0µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Royal jelly and royal jelly lyophilized lincomycin, erythromycin, tilmicosin, tylosin, spiramycin, clindamycin, amphotericin and guitar

Josamycin residue was extracted with Tris extract, the extract was OasisHLB SPE column 1) purification, methanol eluate was concentrated set

After the capacity for liquid chromatography - tandem mass spectrometry. External standard.

1) OasisHLB solid phase extraction columns are Waters company's product trade name, this information is given for the convenience of users of this standard, not the table

Endorsement of the product shown. If other equivalent product has the same effect, you can use these equivalent products.

4 Reagents and materials

Unless otherwise indicated, the reagents used were of analytical grade.