GB/T 22944-2008Determination of clenbuterol residues in honey - LC-MS-MS method (English PDF)

1 Scope

This standard specifies the amount of clenbuterol residues in honey by liquid chromatography - tandem mass spectrometry.

This standard applies to the determination of clenbuterol residues in honey.

The standard method detection limit of 1µg/kg.

2 Normative references

The following documents contain provisions which, through reference in this standard and become the standard terms. For dated references, subsequent

Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research

Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.

GB/T 6379.1 measurement methods and results of Accuracy (trueness and precision) - Part 1. General principles and definitions (GB/T 6379.1-2004, ISO 5725-1.1994, IDT)

GB/T 6379.2 measurement methods and results of Accuracy (trueness and precision) - Part 2. Determine the standard measurement method to repeat

Of the basic methods of reproduction (GB/T 6379.2-2004, ISO 5725-2.1994, IDT)

GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)

Principle 3

Clenbuterol residues in honey with sodium acetate buffer solution (pH = 5.0) extracted, filtered, dried OasisHLB1) or equivalent solid phase

Extraction column, eluted with a solution of ammonia and methanol evaporated and the residue was dissolved in methanol and 0.1% formic acid solution, over 0.2µm membrane, the sample solution

For liquid chromatography - tandem mass spectrometer, internal standard.

4 Reagents and materials

1) OasisHLB solid phase extraction columns are Waters company's product trade name, this information is given for the convenience of users of this standard, not the table

Endorsement of the product shown. If other equivalent product has the same effect, you can use these equivalent products.