GB/T 22506-2008Inspection of grain and oils - Determination of 1- and 2- acyl lysophosphatidylcholine in enzymatically modified phospholipids - High performance liquid chromatography (English PDF)
粮油检验 酶改性磷脂中1-和2-溶血磷脂酰胆碱的测定 高效液相色谱法
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
November 4, 2008
Implementation date
January 20, 2009
Scope
GB/T 22506-2008 is the English-translated version of 粮油检验 酶改性磷脂中1-和2-溶血磷脂酰胆碱的测定 高效液相色谱法.
GB/T 22506-2008 is the Chinese standard "Inspection of grain and oils - Determination of 1- and 2- acyl lysophosphatidylcholine in enzymatically modified phospholipids - High performance liquid chromatography".
Its scope clause reads: This standard specifies the determination of enzyme modified lecithin 1-lysophosphatidylcholine (1-aclylysophosphatidyl- by high performance liquid chromatography choline; 1-LPC) and 2-lysophosphatidylcholine (2-aclylysophosphatidylcholine; 2-LPC) in principle, reagents, instruments provided Equipment, sample preparation, procedures, and results indicate precision and accuracy.
This standard applies to soy lecithin, egg yolk lecithin, rapeseed lecithin as the raw material for preparing the determination of the lysophosphatidylcholine. The standard detection limit of 0.1µg. Its clauses include reagents; equipment.
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Document preview — GB/T 22506-2008
National Standard of the People's Republic of China
- ICS
- 67.040
- Classification
- X 04
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 2 Normative references
- 4 Reagents
- 5 Equipment
1 Scope
This standard specifies the determination of enzyme modified lecithin 1-lysophosphatidylcholine (1-aclylysophosphatidyl- by high performance liquid chromatography choline; 1-LPC) and 2-lysophosphatidylcholine (2-aclylysophosphatidylcholine; 2-LPC) in principle, reagents, instruments provided
Equipment, sample preparation, procedures, and results indicate precision and accuracy.
This standard applies to soy lecithin, egg yolk lecithin, rapeseed lecithin as the raw material for preparing the determination of the lysophosphatidylcholine.
The standard detection limit of 0.1µg.
2 Normative references
Following standard terms of this standard reference and become the standard terms. For dated references, subsequent
Amendments (not including errata content) or revisions do not apply to this standard, however, encourage the parties to the agreement are based on research
Whether the latest versions of these documents. For undated reference documents, the latest versions apply to this standard.
GB/T 6682 analytical laboratory use specifications and test methods (GB/T 6682-2008, ISO 3696. 1987, MOD)
Principle 3
Enzyme modified lecithin products in the 1- and 2-lysophosphatidylcholine and phosphatidylcholine (PC) and other components separated by high performance liquid chromatography,
Using differential refractive index detector and quantified by external standard.
4 Reagents
The following reagents used, unless otherwise specified, are analytical reagents; water is in line with GB/T 6682 provided a water.
4.1
95% ethanol.
4.2
oxalate (H2C2O4 · 2H2O).
4.3
oxalic acid solution (0.02moL/L). Weigh accurately 1.261g of oxalic acid (4.2), dissolved in water and diluted to 500mL.
(2-LPC).
1) standard can be used Sigma's products. Sigma-Aldrich is given only for the convenience of users of this standard, no other commercial activity, other who can
Enough to reach the required standard, you can choose to use.
5 Equipment
5.1 High performance liquid chromatograph
with refractive index detector.
5.2 high-speed centrifuge
10000r/min.
5.3 fiber membrane
0.45µm.
5.4 ultrasonic cleaning
180W.
5.5 Balance
dividing the value of 0.0001g.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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