GB/T 22245-2008Determination of isofraxidin in health foods (English PDF)
保健食品中异嗪皮啶的测定
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Issued by
SAMR; SAC
Level / Type
National · Recommended
Issue date
July 31, 2008
Implementation date
November 1, 2008
Scope
GB/T 22245-2008 is the English-translated version of 保健食品中异嗪皮啶的测定.
GB/T 22245-2008 is the Chinese standard "Determination of isofraxidin in health foods". Its scope clause reads: This standard specifies the method for determination of health food in the isofraxidin.
This standard applies to Ciwujia the main raw material of health food products in different triazine piperidine of measuring skin. When the sampling amount of 2.0g, set the volume to 10mL, injection volume 10µL, the limits of detection (LOD) of 2.0 × 10 -4g/Kg, method The limit of quantitation (LOQ) was 6.0 × 10-4g/kg.
The linear range of 2.0µg/mL ~ 40µg/mL.
Principle 2 According isofraxidin soluble in methanol, acetonitrile, chloroform physical and chemical properties, sample isofraxidin followed by methanol and chloroform two Solutions were extracted methanol to volume, filtered backward high performance liquid chromatography, after reverse phase C18 column by UV detector, according to Retention times and peak areas for qualitative and quantitative. Its clauses include reagents and materials; instruments and equipment.
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Document preview — GB/T 22245-2008
National Standard of the People's Republic of China
- ICS
- 67.040
- Classification
- C 53
Issued by: State Administration for Market Regulation; Standardization Administration of the PRC
Contents
- 1 Scope
- 3 Reagents and materials
- 3.1 methanol (CH3OH)
- 3.2 acetic acid (CH3COOH)
- 3.3 acetonitrile (CH3CN)
- 3.4 chloroform (CHCl3)
- 3.5 Water (H2O)
- 3.6 isofraxidin standard
- 3.8 isofraxidin standard solution (100µg/mL)
- 4 instruments and equipment
- 4.1 High performance liquid chromatography
- 5.1 Sample Processing
- 5.2 Preparation of standard curve
1 Scope
This standard specifies the method for determination of health food in the isofraxidin.
This standard applies to Ciwujia the main raw material of health food products in different triazine piperidine of measuring skin.
When the sampling amount of 2.0g, set the volume to 10mL, injection volume 10µL, the limits of detection (LOD) of 2.0 × 10
-4g/Kg, method
The limit of quantitation (LOQ) was 6.0 × 10-4g/kg. The linear range of 2.0µg/mL ~ 40µg/mL.
Principle 2
According isofraxidin soluble in methanol, acetonitrile, chloroform physical and chemical properties, sample isofraxidin followed by methanol and chloroform two
Solutions were extracted methanol to volume, filtered backward high performance liquid chromatography, after reverse phase C18 column by UV detector, according to
Retention times and peak areas for qualitative and quantitative.
3 Reagents and materials
3.1 methanol (CH3OH)
pure class distinctions.
3.2 acetic acid (CH3COOH)
AR.
3.3 acetonitrile (CH3CN)
chromatography.
3.4 chloroform (CHCl3)
AR.
3.5 Water (H2O)
a laboratory water conductivity (25 °C) of 0.01mS/m.
3.6 isofraxidin standard
Purity >=99%.
3.7
isofraxidin stock standard solution (1.00mg/mL). Weigh accurately isofraxidin standard 0.01g (accurate to 0.0001g), placed
10mL volumetric flask, and dissolved in methanol to volume, and mix (this standard solution at 4 °C refrigerator to save 7d).
3.8 isofraxidin standard solution (100µg/mL)
lessons isofraxidin stock standard solution 1.00mL to 10mL volumetric flask, methanol
To volume, and mix (this standard solution at 4 °C refrigerator to save 7d).
4 instruments and equipment
4.1 High performance liquid chromatography
a UV detector or diode array detector.
4.2
ultrasonic cleaner.
Step 5 Analysis
5.1 Sample Processing
According to the content of the sample, weighed 0.5g ~ 5g homogeneous sample (accurately weighed to 0.001g) placed in 25mL test tube, add methanol 20mL,
After ultrasonic extraction 20min, filtered, washed with 20mL methanol and the residue was washed three times, collect all the filtrate evaporated to dryness in a water bath, add appropriate amount of water-soluble
Solution and transferred to a separatory funnel with 30mL chloroform at the second, and the combined chloroform extracts were evaporated to dryness in a water bath, add methanol
Dissolved and set the volume to 2.0mL, through 0.45µm membrane, and the filtrate set aside.
5.2 Preparation of standard curve
Use standard solution was diluted with methanol to prepare a standard solution isofraxidin concentrations were 2.00µg/mL, 4.00µg/mL,
8.00µg/mL, 10.0µg/mL, 20.0µg/mL, 40.0µg/mL as the standard series.
......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 15 pages — is available in the English PDF.
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