GB/T 21826-2025Chemicals — Test method of acute oral toxicity — Up-and-down procedure (UDP) (English PDF)

Foreword

This document complies with the provisions of GB/T 1.1-2020 "Standardization Work Guidelines Part 1: Structure and Drafting Rules of Standardization Documents". Drafting.

This document replaces GB/T 21826-2008 "Acute Oral Toxicity Test Methods for Chemicals - Dosage Increase/Decrease Method (UDP)".

Compared with GB/T 21826-2008, apart from structural adjustments and editorial changes, the main technical changes are as follows.

a) The requirements for animal selection have been changed (see 5.1.1.1, 2008 version 5.1.1.1);

b) The husbandry conditions have been changed (see 5.1.2, 2008 version 5.1.2);

c) The classification requirements for the 2000 mg/kg dose have been changed (see Appendix A, Appendix C of the 2008 edition).

Please note that some content in this document may involve patents. The issuing organization of this document assumes no responsibility for identifying patents.

This document was proposed and is under the jurisdiction of the National Technical Committee on Standardization of Hazardous Chemicals Management (SAC/TC251).

This document was drafted by: Shenzhen Customs Industrial Products Testing Technology Center and Ningbo Inspection and Quarantine Science and Technology Research Institute (Ningbo International Trade Convenience Center).

(Lihua Service Center), Ningbo Customs Technology Center.

The main drafters of this document are: Wu Jingwu, Yu Weiteng, Liu Dong, Sun Yun, Chen Junbin, Wu Shuying, Zhang Xiaoxian, Lu Yu, Liu Hanwei, and Li Yingzhou. Feng Junli and Chen Youwei.

The release history of this document and the document it replaces is as follows.

— First published in 2008 as GB/T 21826-2008;

— This is the first revision.

Introduction

The up-and-down dosing method was first proposed by Dixon and Mood. In 1985, Bruce suggested using the up-and-down dosing method (UDP).

The experimental design for estimating LD50 using the incremental dosing method for determining the acute toxicity of chemicals has undergone several revisions. This document is based on Bruce's work.

Materials (ASTM) adopted it and revised it in 1990.In 1995, it was published using UDP, the conventional LD50 test method, and...

The results of studies using three methods in the fixed-dose method. Starting with the early papers by Dixon and Mood, many studies have used these methods to obtain results.

Research on component optimization has appeared in biological and applied papers. Several expert meetings held in 1999 gave the following recommendations regarding this method.

In fact, a consensus has been reached on the unified use of LD50 for classifying chemicals; it is generally believed that the sex of model organisms (usually) It is sufficient to test females; to make the estimates more meaningful, confidence intervals (CIs) need to be estimated.

Acute oral toxicity test methods for chemicals Dosage adjustment method (UDP)

1 Scope

This document describes the dosing-up-down method (UDP) for acute oral toxicity testing of chemicals.

This document applies to the use of UDP for testing the acute oral toxicity of chemicals. This method is more suitable for use within one or two days after exposure.

The substance that causes death.

This document does not apply to substances that may significantly delay the expected time to death (5 days or more) after exposure.

2 Normative references

This document has no normative references.

3 Terms and Definitions

The following terms and definitions apply to this document.