GB/T 20758-2006Method for the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues - LC-MS-MS method (English PDF)

1 Scope

This standard specified the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues by LC-MS-MS method.

This standard is applicable to the determination of testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues.

Method detection limits of this standard: in bovine liver, testosterone, epi-testosterone and progesterone: 0.5 µg/kg; in muscle tissues, testosterone and epi-testosterone: 0.1 µg/kg, and progesterone: 0.5 µg/kg.

2 Normative references

The following normative documents contain provisions which, through reference in this text, constitute provisions of this standard. For dated references, subsequent amendments (excluding corrections), or revisions, of any of these publications do not apply to this standard. However parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent editions of the normative documents indicated below. For undated references, the latest edition applies.

GB/T 6379.1 Accuracy (trueness and precision) of measurement methods and results - Part 1: General principles and definitions ( GB/T 6379.1-2004, ISO 5725-1:1994, IDT)

GB/T 6379.2 Accuracy (trueness and precision) of measurement methods and results - Part 2: Basic method for the determination of repeatability and reproducibility of a Standard measurement method (GB/T 6379.2-2004, ISO 5725-2:1994, IDT)

GB/T 6682 Water for analytical laboratory use-specification and test methods (GB/T 6682-1992, neq ISO 3696: 1987)

3 Principle

After enzymolysis, the testosterone, epi-testosterone and progesterone residues in bovine liver and muscle tissues are extracted with methanol and methyl tert-butyl ether; the extracting solution is purified with C18 solid phase extraction column, while the liver sample is purified again with silica gel solid phase extraction column; the eluent is concentrated to constant volume for determination by LC-MS-MS method.