GB/T 18654.15-2008Inspection of germplasm for cultured fishes -- Part 15: RAPD analysis (English PDF)

Foreword

GB/T 18654 "Inspection of germplasm for cultured fishes" is divided into the following sections.

--- Part 1. Inspection rules;

--- Part 2. Sampling methods;

--- Part 3. Determination of traits;

--- Part 4. Determination of age and growth;

--- Part 5. Diet analysis;

--- Part 6. Determination of reproductive performance;

--- Part 7. Ecological Characteristics;

--- Part 8. Determination of the oxygen consumption rate and the critical point of suffocation;

--- Part 9. Includes meat for determination;

--- Part 10. Determination of muscle nutrients;

--- Part 11. Determination of the major muscle amino acid contents;

--- Part 12. karyotype analysis;

--- Part 13. isoenzyme electrophoresis analysis;

--- Part 14. Determination of DNA content;

--- Part 15. RAPD analysis;

This is Part 15 GB/T 18654 of.

This section of the Appendix A, Appendix B and Appendix C are normative appendix.

This section proposed by the People's Republic of China Ministry of Agriculture.

This part of the National Standardization Technical Committee of aquatic freshwater aquaculture division Technical Committee.

This section is drafted. Shanghai Fisheries University, Chinese Academy of Fishery Sciences, Yangtze River Fisheries Research Institute.

1 Scope

This section GB/T 18654 specifies the fish RAPD analysis reagents and materials, instruments and equipment, sampling, analysis step and results determination.

This section applies to common freshwater aquaculture fish.

2 Normative references

The following documents contain provisions which, through reference GB/T 18654 in this section constitute provisions of this section. For dated reference documents Member, all subsequent amendments (not including errata content) or revisions do not apply to this section, however, encouraged to reach under this section Parties to research agreement to use the latest versions of these documents. For undated reference documents, the latest versions apply to this section.

GB/T 18654.1-2002 test germplasm for cultured fishes - Part 1. Inspection Rules GB/T 18654.2 Inspection of germplasm for cultured fishes - Part 2. Sampling Method Principle 3 with a 10 base random oligonucleotide primers for genomic DNA sample was amplified by PCR amplification products were separated by electrophoresis and ethidium bromide staining, according to the band Polymorphism analysis, determination of genetic characteristics of fish species.

4 Reagents and materials

Unless otherwise indicated in the analysis using only recognized as analytical grade reagents and distilled or deionized water or equivalent purity.

4.1 Tris (Tris).

4.2 Sodium hydroxide (NaOH).

4.3 Sodium chloride (NaCl).

4.4 sodium dodecyl sulfate (SDS).

4.5 potassium chloride (KCl).

4.6 Magnesium chloride (MgCl2).

4.7 Disodium ethylenediaminetetraacetate (EDTA).

4.8 boric acid.

4.9 agarose.

4.10 sucrose.

4.11 gelatin.

4.12 ethidium bromide (EB).

4.13 proteinase K.

4.14 RNA enzyme A (no DNA activity).

4.15 T Rao q enzyme.

4.16 DNAMarker.

4.17 paraffin oil.