GB 28146-20113% Gibberellic acid emulsifiable concentrates (English PDF)

3.1 Composition and appearance

This product should meet the standards of the original drug made of gibberellic acid, a stable homogeneous oily liquid without visible suspended matter and sediment.

3.2 Technical Specifications

3% gibberellic acid EC shall comply with the requirements of Table 1.

Table 1 3% gibberellic acid EC Control Project Index

Item Index

Gibberellic acid mass fraction /% 3.0 0.3-0.3 Moisture content /% <= 5.0 a pH range of 2.5 to 4.5 Emulsion stability (diluted 200 times) Qualified Low temperature stability a qualified Thermal storage stability of a qualified a low temperature stability test, thermal storage stability test in the normal production and testing at least once every three months.

Test Method 4

Safety Tips. Use of this standard shall have practical experience of laboratory personnel work. This standard does not point out all of the safety issues. Make with those who have the responsibility to take appropriate safety and health practices and to ensure compliance with the relevant national regulations.

4.1 General provisions

This standard reagents and water in the absence of other requirements specified, refers to three analytical reagent and GB/T 6682-2008 specified in water. Test results determined in accordance with GB/T 8170-2008 4.3.3 Rounding the value comparison method.

4.2 Sampling

According to GB/T 1605-2001 in "liquid formulations sampling" approach. Random sampling method is determined by the package, the final sample size should not be Less than 200mL.

4.3 Identification Test

The identification test can be carried out simultaneously with the determination of the mass fraction of gibberellic acid. Under the same chromatographic operating conditions, the main sample solution chromatogram Retention time and peak standard solution of gibberellic acid chromatographic peak retention time, the relative difference should be less than 1.5%.

4.4 Determination of the mass fraction of gibberellic acid

4.4.1 Method summary

Sample dissolved in mobile phase, methanol - phosphoric acid as the mobile phase, use as filler to C18 stainless steel column and UV detector, for Gibberellic acid in the sample by reverse phase HPLC separation and determination of external standard.

4.4.2 Reagents and solutions

Phosphoric acid;

Methanol. HPLC grade;

Water. The new secondary steam distilled water;

Phosphoric acid. [Psi] (water. phosphoric acid) = 2000.1;

Gibberellic acid standard sample. a known mass fraction w>=98.0%.

4.4.3 Instruments

High performance liquid chromatography. a variable wavelength UV detector and quantitative injection valve;

Chromatographic data processor or chromatography workstation;

Column. 250mm × 4.6mm (id) stainless steel column, built ZORBAXEXTENDC18,5µm filler (or with the same Other effects such as columns);

Micro injector. 100µL;

Filter. pore size of about 0.45µm;

Ultrasonic cleaner.

4.4.4 HPLC operating conditions

Mobile phase. [Psi] (methanol. aqueous phosphoric acid solution) = 35.65;

Flow rate. 1.0mL/min;

Column temperature. room temperature (temperature change should not exceed 2 °C);

Detection wavelength. 210nm;

Injection volume. 10µL.

Retention time. gibberellic acid about 8.2min.

HPLC operating conditions described above, the Department of typical operating parameters, according to the characteristics of different instruments, given operating parameters to make appropriate adjustments to to obtain the best results. A typical 3% gibberellic acid cream HPLC is shown in Figure 1.

1 --- gibberellic acid.

1 3% gibberellic acid by high performance liquid chromatogram EC

4.4.5 Measuring step

4.4.5.1 Preparation of standard solution

Weigh gibberellic acid standard about 0.05g (accurate to 0.0002g), placed in 100mL flask, add 35mL of methanol to dissolve, then Phosphoric acid aqueous solution was diluted to the mark.

4.4.5.2 Preparation of the sample solution

Weigh a sample containing about 0.05g of gibberellic acid (accurate to 0.0002g), placed in 100mL flask, add 35mL of methanol to dissolve, Phosphoric acid aqueous solution and then diluted to the mark. Filtered through a 0.45µm pore size filters.

4.4.5.3 Determination

Under these operating conditions, after the instrument is stable, continuous injection of several doses of the standard solution until the two adjacent needles gibberellic acid peak area relative change After less than 1.5%, according to the solution, sample solution, sample solution, standard order of standard solution was measured.

4.4.6 Calculation

The two needle sample solution and measured before and after the two doses of the standard sample solution of gibberellic acid peak area were averaged. Sample gibberellic acid Mass fraction w1 (%) according to equation (1).

w1 = A2

· M1 · w

A1 · m2

Where.

A2 --- sample solution, gibberellic acid, the average peak area;

m1 --- gibberellic acid standard sample mass in grams (g);

w --- Sample mass fraction of gibberellic acid,%;

A1 --- standard solution, gibberellic acid, the average peak area;

m2 --- sample mass, in grams (g).

4.4.7 Allowable difference

The difference between two parallel determination results shall not be greater than 0.2%, the arithmetic mean value as a measurement result.

4.5 Determination of Moisture

According to GB/T 1600 the "Karl Fischer method".

Determination of 4.6 pH value According to GB/T 1601 carried out.

4.7 Emulsion stability test

According to GB/T 1603 test. No oil slick in the cylinder (cream), heavy oil and precipitated as qualified.

4.8 Low temperature stability test

According to GB/T 19137 in "homogeneous emulsion and liquid preparations", in the bottom of the centrifuge tube educt volume does not exceed 0.3mL qualified.

4.9 Thermal storage stability test

According to GB/T 19136 in "liquid formulation" to. After the thermal storage, gibberellic acid content shall not be less than 95% before the heat storage mass fraction; milk Liquid stability should still meet the standards.

4.10 Product inspection and acceptance

Shall comply with GB/T 1604's.

5.1 Mark, label

3% gibberellic acid EC marking, labeling and packaging should comply with the provisions of GB 4838.

5.2 Packaging

3% gibberellic acid application with the EC within the plug and cover brown glass bottle or PET bottle packaging, bottle net content of 100mL (g), 200mL (g), 500mL (g); overpack Plastic boxes or corrugated boxes, each box net content not exceeding 15kg. According to user requirements or set Goods agreement, the use of other forms of packaging, it should comply with the provisions of GB 4838.

5.3 Storage

3% gibberellic acid cream package should be stored in ventilated, cool, dry coffers, fire, moisture, and can not be in the open air with toxic substances, Flammable material and alkaline material stacked together. Not with food, seeds, feed mixed release.

Storage and transportation to prevent moisture and sun, and rain to avoid collision, away from fire.

5.4 Security

This product is a low toxicity plant growth regulator. The FDA should wear protective gloves, masks, wearing clean protective clothing. When you can not use with a base Items mixed, not be mixed with ammonia, calcium polysulfide, soda ash and the like. Used with the distribution, once dubbed the aqueous solution can not go far. Shall immediately after use Wash with water. In case of poisoning, should go to hospital for treatment.

5.5 Warranty Period

Under the specified conditions of storage, 3% of gibberellic acid to ensure EC, two years from date of manufacture.

Appendix A

(Informative)

Other names gibberellic acid, structural formula and basic physicochemical parameters this product is an active ingredient gibberellic acid is another name, structural formula and basic physicochemical parameters are as follows.

ISO common name. Gibberelicacid CIPAC Numeric Code. 307 CAS Registry Number. 77-06-5 Chemical name. (1alpha, 2beta, 4aalpha, 4bbeta, 10beta) -2,4a, 7- trihydroxy-1-methyl-8-methyl-3-ene-1,10-gibberellic acid -1 , 4a-Lactone Structure.

Empirical formula. C19H22O6 Molecular Weight. 346.4 Biological activity. Plant Growth Regulator Melting point. 223 °C ~ 225 °C (decomposition) Solubility. water 5g/L (room temperature). Soluble in methanol, ethanol, acetone, alkaline aqueous solution, slightly soluble in diethyl ether and ethyl acetate, insoluble Chloroform. Its potassium, sodium, ammonium salts soluble in water (potassium salt 50g/L) Stability. At room temperature in a dry state is stable, slow degradation in aqueous or aqueous alcohol solution in, DT50 (20 °C) about 14d (pH3 ~ 4), 14d (pH7), alkali metal will be converted into less biologically active compounds, the case of thermal decomposition.