YY/T 1529-2017ELISA analytical instruments (English PDF)

5.2.6 Sensitivity

Use the sensitivity concentration standard substance of concentration 5 mg/l for the

ELISA analytical instruments; and the measured absorbance value of the instruments shall be not less than 0.01.

5.2.7 Difference of channels

Using the air as the reference, measure the absorbance difference between 8 channels;

and the required result shall not be greater than 0.02.

5.3 Electrical safety requirements

As specified in the applicable clauses of GB 4793.1 and YY 0648.

5.4 Environmental test requirements

As specified in the applicable clauses of GB/T 14710.

5.5 EMC requirements

As specified in the applicable clauses of GB/T 18268.1 and GB/T 18268.26.

5.6 Software functions

The ELISA analytical instruments shall, at least, have the following functions:

a) having the data display function;

b) being capable of setting up test items, test methods and test dates flexibly;

c) being capable of continuously storing the test items and results in succession;

d) being capable of displaying and printing the test items, test data and test time.

6.1 Operating conditions

6.1.1 Power supply requirements: voltage 220 V ± 22 V (a.c.); frequency 50 Hz ± 1

Hz.

6.1.2 Environmental temperature: + 10°C ~ +40°C.

6.1.3 Relative humidity: 30% ~ 80%.

6.1.4 The following items shall be carried out after the instrument is started and stabilized for 30 min. When the following experiments are carried out on the instrument,

Annex A (Informative)

Preparation Method of Sensitivity Solution Standard Substance for the

ELISA Analytical Instruments

For the sensitivity solution standard substance for the ELISA analytical instruments, use pure substance of potassium dichromate and sulfuric acid 0.05 mol/l as the matrix to prepare the solution standard substance 5 mg/l.

A.1 Preparation of sulfuric acid solution 0.05 mol/l

Add 400 ml of distilled water into a 500 ml beaker; use a pipette to absorb 2.7 ml of concentrated sulfuric acid into the beaker; stir up before pouring into a 1 000 ml volumetric flask; add distilled water to dilute to the scale line; and shake up as standby.

A.2 Preparation of potassium dichromate solution standard substance of

chromium content 5 mg/l

A.2.1 Step 1

Weigh accurately 0.282 9 g of potassium dichromate on the analytical balance to place into a 100 ml beaker; use sulfuric acid solution 0.05 mol/l to dissolve before transferring to a 500 ml volumetric flask; use a small amount of sulfuric acid solution 0.05 mol/l to wash the beaker for several times; pour the washings into the volumetric flask; use sulfuric acid solution 0.05 mol/l to dilute to the scale line; and shake up as standby.

The concentration of the solution is 200 mg/l.

A.2.2 Step 2

Use a pipette to transfer 2.5 ml of potassium dichromate solution 200 mg/l into a 100 ml volumetric flask; use sulfuric acid solution 0.05 mol/l to dilute to the scale line; and shake up. Then, the concentration of the solution is 5 mg/l.