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WS 589-2018Bio-safety signs for pathogenic microorganism laboratory (English PDF)

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Issued by

National Health Commission

Level / Type

National · Mandatory

Issue date

March 6, 2018

Implementation date

August 1, 2018

Scope

WS 589-2018 (Bio-safety signs for pathogenic microorganism laboratory) is available as an English-translated PDF.

WS 589-2018 — This standard specifies the standard setting, operation, maintenance and management of the biosafety label of the pathogenic microorganism laboratory.

This standard applies to research, teaching, testing, diagnosis, preservation and biological systems related to pathogenic microorganisms (poisons) and samples. A laboratory for related activities such as production.

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Document preview — WS 589-2018

National Standard of the People's Republic of China

ICS
11.020
Classification
C 59

Issued by: National Health Commission

Contents

  • Foreword
  • 1 Scope
  • 2 Normative references
  • 3 Terms and definitions
  • 4 Identification type
  • 4.1 Prohibition of identification
  • 4.2 Warning Sign
  • 4.3 Instruction ID
  • 4.4 Prompt identification
  • 4.5 Special identification
  • 5 Identification requirements
  • 5.1 Chroma performance
  • 5.2 Font Requirements

Foreword

The full technical content of this standard is mandatory.

This standard was drafted in accordance with the rules given in GB/T 1.1-2009.

This standard refers to the International Organization for Standardization ISO 7010 Graphical symbols-Safety colours and safety signs (graphic symbols -Safety color and safety mark), combined with the current national standard GB 2894-2008 "Safety Mark and its Usage Guidelines", GB/T 2893-2004 "Graphic Symbol Safety Colors and Safety Labels Part 1. Design Principles for Safety Marks in Workplaces and Public Areas" quasi.

This standard was drafted. China Center for Disease Control and Prevention, Chinese Center for Disease Control and Prevention, China Center for Disease Control and Prevention, China Medical Institute of Pathogenic Biology, Institute of Medical Laboratory Animals, Chinese Academy of Medical Sciences, China Center for Disease Control and Prevention, Prevention and Control of Infectious Diseases Institute, China Medical University.

1 Scope

This standard specifies the standard setting, operation, maintenance and management of the biosafety label of the pathogenic microorganism laboratory.

This standard applies to research, teaching, testing, diagnosis, preservation and biological systems related to pathogenic microorganisms (poisons) and samples.

A laboratory for related activities such as production.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only the dated version applies to this document.

For undated references, the latest edition (including all amendments) applies to this document.

GB 2893 (all parts) safety color GB 2894 safety signs and guidelines for their use GB 12268-2012 Dangerous Goods Name List GB/T 14691 technical drawing font GB/T 15566 (all parts) Principles and requirements for setting up public information guidance systems GB 15630 fire safety sign setting requirements

3 Terms and definitions

The following terms and definitions apply to this document.

Safety sign

An identifier used to express specific security information, consisting of graphical symbols, security colors, geometric shapes (borders), or text.

Safe color

The color that conveys the meaning of the security information, including red, blue, yellow, and green.

Contrast color

A more striking contrast color for safe colors, including black and white.

Prohibition sign prohibition sign Graphical signs prohibiting people from unsafe behavior.

Warning sign

Remind people to pay attention to the surrounding environment to avoid dangerous graphic signs.

Instruction sign

Emphasize the graphical signs that people must make certain actions or take precautions.

Prompt identification information sign A graphic mark that provides people with certain information (such as a safety facility or location).

Description sign

A sign that provides specific instructions (marked as a safety classification or protective measure, etc.), consisting of geometric borders and text.

Special mark

A symbol or marker made for a particular thing, product, or device that is labeled for easy identification.

3.10

Environmental information sign the information provided relates to graphical identifiers for larger areas. Identification type code. H.

3.11

Partial information sign the information provided relates only to the location of a location or even the graphical identity of a device or component. Identification type code. J.

3.12

Luminance factor

Under specified lighting and viewing conditions, the brightness of a given point on a surface of a non-self-illuminating body is completely reflected or under the same conditions the ratio of the brightness of a fully transmissive diffuser.

3.13

Ordinary material

A material that does not retroreflect light and does not emit light.

3.14

Retroreflective material A material whose direction of light reflection is close to the opposite direction of incidence of light.

3.15

Combined material

A material that combines the optical characteristics of a photoluminescent material with a retroreflective material.

4 Identification type

4.1 Prohibition of identification

Forbidden signs are shown in Table 1.

Table 1 prohibition of identification

Number graphic identifier name Identification kind Setting range and location 1-1 no Entry no entering Workplaces that can cause occupational hazards at or near the mouth of the danger zone, if possible Biohazard equipment failure, maintenance, Equipment and facilities for overhauling biohazards when setting according to the actual situation on site 1-2 no entry no thoroughfare H, J Dangerous work areas, such as laboratories, sewage Dyeing source, etc.

Children are not allowed inside no children will be Admit a field that is prone to accidents or injuries to children such as laboratory areas, various sources of pollution Area, etc.

Number graphic identifier name Identification kind Setting range and location 1-4 no pets allowed no pets Pets entering the area carry infectious diseases Bacteria, a place that is easy to cause harm to people, such as laboratory areas, various sources of pollution 1-5 no smoking no smoking Laboratory, no smoking place, truthful Laboratory area, carbon dioxide storage area and Hospital, etc.

Ban fireworks

No burning

Laboratory flammable and explosive chemicals are stored and made Use and laboratory operating area 1-7 no open flames no open flames H, J Laboratory flammable and explosive chemicals are stored and made Use and laboratory operating areas, such as fume hoods, Fume hoods, drug storage cabinets, etc.

Prohibition of carrying jewelry, metal Object or watch no metallic Accessories Sites where experimental activities are carried out, such as. experiments Entrance to the room or entrance to the room 1-9 no stacking no stocking Fire equipment storage, fire exit, and Portable eye washers and emergency sprinklers nearby Number graphic identifier name Identification kind Setting range and location 1-10 Prohibit closing no switching on when the equipment or line is overhauled, the corresponding switch is attached.

1-11

Prohibit water from extinguishing no extinguishing with water H, J Storage and transportation, use of water to extinguish fire Quality sites, such as transformer rooms, laboratory cores Heart area and precision instruments, etc.

1-12

Prohibit close

No nearing

Dangerous areas that are not allowed to be approached, such as substation Equipment, high-level biosafety laboratory Near the spare room, etc.

1-13

Prohibit promotion

No pushing

A device or device that is easy to dump, such as a gas Cylinders, precision instruments, etc.

1-14

No touch

Blowing

Prohibited to touch nearby devices or objects, such as Laboratory power control box, pressure steam off Surface of the autoclave process, liquid nitrogen, and toxic, corrosive objects, etc.

1-15

No gloves to touch

No with

Glove

It is forbidden to wear contaminated (pathogenic microorganisms) Gloves for touching instruments and supplies Number graphic identifier name Identification kind Setting range and location 1-16 no photo or video recording no photos Do not shoot or use flash according to management requirements Lights can easily affect experimental activities or cause instruments Bad consequences such as equipment and personnel light damage Place 1-17 Do not open wireless mobile communication Information equipment no activated mobile Phones Using wireless mobile communication equipment is easy to cause explosion Explosion, combustion and electromagnetic interference and leaking fields 1-18 Do not use mouth to aspirate no sucking liquid J. It is forbidden to pipette by mouth suction.

1-19

No diet

No food or drink

a place that is easy to cause personal injury, truthful Laboratory area, entrance to pollution source, medical treatment Garbage storage, operating room, etc.

1-20

No food storage

No food storage

Areas or places where food is prohibited, such as Laboratory area, entrance to pollution source, medical Treatment of garbage storage and operating rooms, etc.

1-21

Prohibit littering

No littering

Throw the waste to a designated location or container Inside, such as sharp box, medical garbage bag and finger in a fixed container Number graphic identifier name Identification kind Setting range and location 1-22 Do not open no opening Laboratory that is prohibited from opening due to work needs 1-23 Prohibit startup no starting Suspended instruments and implementation equipment attached Near, such as instrument overhaul and parts replacement Related places 1-24 Fatigue work no fatigue work it is forbidden to open when fatigue and immunity are low.

Exhibition work, such as fever, cough, full when the body is weak and other symptoms

4.2 Warning Sign

The warning signs are detailed in Table 2.

Table 2 Warning signs

Number graphic identifier name Identification kind Setting range and location 2-1 Biological hazard Biohazard H, J Sites susceptible to infection, such as biosecurity Secondary and above laboratory entrance, bacteria (poison) and the entrance to the sample collection and infection Surface of a transport container for a substance 2-2 be careful Warning danger J Places and equipment that cause personal injury Number graphic identifier name Identification kind Setting range and location 2-3 Beware of fire Warning fire H, J a dangerous place that is prone to fire, such as an experiment Ventilation for storage and use of flammable substances Kitchen, fume hood, chemical reagent cabinet, etc.

Beware of explosion

Warning explosion

a place prone to explosion hazard, such as an experiment Stores flammable and explosive materials, flammable and easy Explosive substance use or pressurized container storage place 2-5 Beware of corrosion Warning Corrosive substances (GB 12268-2012) Site of the substance specified in category 8) Points such as reagent chamber, dosing chamber, and washing chamber 2-6 Beware of chemical burns Beware of chemical Burns Storage and use of corrosive chemicals 2-7 Beware of poisoning Warning poisoning H, J Drugs and toxic substances (GB 12268-What is specified in Category 6, Item 1 of 2012 Storage and use place, such as reagents Cabinet, toxic handling office 2-8 Beware of electric shock Warning electric Shock Electrical equipment that may present a risk of electric shock and lines, such as power distribution rooms, switches, etc.

Number graphic identifier name Identification kind Setting range and location 2-9 Beware of automatic start Warning automatic Start-up J Equipment with automatic starter 2-10 Watch your head Warning overhead Obstacle J is easy to produce a place to meet, such as equipment interlayer 2-11 Beware of hurting hands Warning sharp Objects Laboratory slicing and other operations are likely to cause hand injuries Dangerous work place 2-12 Beware of hot surfaces Warning hot surface a place or object table with a hot object surface Surface, such as autoclave, pressure steam Bacteria, drying oven, etc.

2-13

Beware of low temperatures Warning low Temperature/Freezing Conditions Places that are prone to frostbite, such as cold storage, Gasifier surface, field with liquefied gas Liquid nitrogen, etc.

2-14

Beware of slipping

Warning slippery

Surface

Ground that is prone to slip damage, such as high Biosafety laboratory shower, reagent Residual night, disinfectant, etc.

Its accident handling process) Number graphic identifier name Identification kind Setting range and location 2-15 Beware of high pressure vessels Warning high Pressure vessel H, J a field prone to explosion and injury of pressure vessels such as carbon dioxide cylinders, high (and/or Low) pressurized liquid nitrogen tank and pressure steam sterilizer 2-16 Beware of ultraviolet rays Warning ultraviolet UV-induced various injuries Sites such as biosafety cabinets, clean benches, and UV disinfection in the core area of the laboratory 2-17 Beware Warning sharp Objects Items that are prone to skin punctures and cuts or workplace, such as chicken embryo inoculation, bacteria (poison) Freeze-drying preservation process 2-18 Beware of splash Warning splash a field with liquid and aerosol spills as in the process of treating infectious substances Use homogenizer, ultrasonic, centrifuge, etc.

2-19

Beware of animal damage Warning animals may bite There may be animal attacks during the experiment (eg Animal bites, scratches, etc.) cause injury Harmful place 2-20 Beware of ionizing radiation Caution isotope Tion H, J can produce isotope and ionizing radiation hazards Workplace Number graphic identifier name Identification kind Setting range and location 2-21 Hazardous Waste Hazardous waste H, J Hazardous waste storage and disposal sites, such as Sheng Container surface containing infectious substances, harmful Production, storage and use of biological products

4.3 Instruction ID

The instruction identification is detailed in Table 3.

Table 3 instruction identification

Number graphic identifier name Identification kind Setting range and location 3-1 Must wear protective clothing Must wear Protective clothes Wear protective clothing to prevent personal infection a place, such as a laboratory entrance or a dressing room Entrance 3-2 Must wear overalls Must wear work Clothes Work clothes must be worn as required (laboratory basis) the place where the work clothing), such as the laboratory Low risk, no need to wear protective clothing General work area 3-3 Must wear a protective cap Must wear Protective cap J Experimental area that easily pollutes the human head 3-4 Must wear goggles Must wear Protective goggles J Workplace that is harmful to the eyes Number graphic identifier name Identification kind Setting range and location 3-5 Must wear a mask Must wear Protective face Shield Gas harmful to the human body and easy to produce aerosol Glue place 3-6 Must wear a breathing apparatus Must wear breathing Apparatus Risk assessment, easy to lead to respiratory infections, High level biosafety requiring appropriate protection Laboratory, such as facial and respiratory defense Protected area 3-7 Must wear a disposable mask Must wear Disposable masks Preventing the splashing of pathogenic substances in the laboratory when, for example, centrifugation, homogenizer Homogenization process, etc.

Must wear a mask (N95 and Upper model) Must wear mask (N95 or higher level) Operation of the pathogenic microbial name of human infection Record" (Wei Ke Jiao Fa [2006]] No. 15) "Biosafety experiment required for experimental activities" a place specified at the room level, such as biosafety all three-level laboratory, animal biosafety three Laboratory and above 3-9 Must wear ear protectors Must wear ear Protector J workplace with noise exceeding 85 dB 3-10 Must wear protective gloves Must wear Protective gloves Workplaces that are susceptible to hand infections and injuries as an infectious substance, it has rot Eclipse, pollution, burning, freezing and electric shock Risky work Number graphic identifier name Identification kind Setting range and location 3-11 Must wear a shoe cover Must wear shoe Covers Easy to cause foot pollution and spread pollution Industry sites, such as laboratory core work rooms, etc.

location

3-12

Must wear protective shoes Must wear Protective shoes Workplaces that are prone to foot infections and injuries such as corrosion, pollution, sputum (thorn) Dangerous work locations such as injuries 3-13 Must wash your hands Must wash your Hands Hand on the pathogenic microorganism after the experiment Clean equipment or supplies, such as special water Near the pool 3-14 Must be disinfected by hand Must disinfect Hands End of the experimental activity in the biosafety laboratory After killing the pathogens that may be carried on the hands biological 3-15 Must be locked Must be locked Drugs, dangerous goods and pathogenic substances in places such as warehouses, such as placing infectious substances Refrigerator, freezer, sample cabinet, toxic Harmful, flammable and explosive storage place 3-16 Must be fixed Must be fixed Fixed to prevent movement or dumping Measures near objects such as carbon dioxide steel Bottle, high (and/or low) pressurized liquid nitrogen tank storage Number graphic identifier name Identification kind Setting range and location 3-17 Must be aired Must be Ventilated H, J Produces toxic and harmful chemical gases, pathogenicity Biological factor aerosol site

4.4 Prompt identification

The prompts are detailed in Table 4.

Table 4 prompt identification

Number graphic identifier name Identification kind Setting range and location 4-1 emergency exit Emergent exit an emergency exit that facilitates safe evacuation, and the direction arrow is combined with the emergency exit Channels, stairways, etc. See GB 4-2 Crushing the board Break to obtain Access Must open the board to get the exit, such as Emergency escape exit, fire alarm panel, etc.

Number graphic identifier name Identification kind Setting range and location 4-3 First aid point First aid Set up on-site first aid equipment and medicines location 4-4 Emergency call Emergency Telephone J Location where emergency telephone is installed 4-5 Eye wash device Eyewash station Place the emergency eyewash device, such as washing Near the eye 4-6 Biosafety emergency response box Biosafety Emergency box Place a biosafety accident emergency treatment the location of the item, such as a biosafety emergency box nearby 4-7 Toolbox Tool box J Laboratory instrument repair tool storage Number graphic identifier name Identification kind Setting range and location 4-8 Animal experiment Animal experiment in the laboratory, in order to get the relevant creatures Knowledge of medicine and medicine, and use a place for scientific research 4-9 Emergency spray Emergency spray Set the location of the emergency sprinkler, such as spraying Near the shower or sprinkler 4-10 Disinfection Disinfecting Prompt disinfection is in progress, as is ongoing Disinfected area and laboratory entrance

4.5 Special identification

The special logo is shown in Table 5.

Table 5 special identification

Number graphic identifier name Identification kind Setting range and location 5-1 Biological hazard Biohazard Place biosafety laboratory entrance Different levels of biosafety experiments the room has corresponding labels, such as bio-an Full three-level laboratory mark "BSL-3" Number graphic identifier name Identification kind Setting range and location 5-2 equipment status Equipment status in normal use, suspended use, Discontinued instrument and equipment on or near the equipment 5-3 Medical waste Medical waste H, J Medical waste generation, transfer, storage and may cause danger during disposal Surface of a damaged item, such as medical waste Disposal center, medical waste temporary storage room and medical waste disposal facilities nearby and the surface of medical waste containers, etc.

At work

In the work

Need to show that the lab is working a prominent position of the state, such as a laboratory Main entrance or protected area entrance, etc.

(can be used in light boxes) Note. In addition to the safety instructions mentioned above, the laboratory and other places related to the laboratory must also indicate the corresponding safety.

Markings, such as the use of chemical hazard signs and warning lines, should be in accordance with the corresponding requirements.

5 Identification requirements

5.1 Chroma performance

All colors such as characters, symbols, borders and substrates of the logo surface shall comply with the provisions of GB 2893 on the color range of materials, common materials, the coordinates and brightness factors of the various colors of the retroreflective light material and the fluorescent material are shown in Figures 1 and 6, respectively.

The range of safe color chromaticity that meets the exact color requirements shall be in accordance with Table 7.

When the coordinate values of the corners of various chromaticity of the security color deviate from the range specified by the chromaticity diagram, the color should not be used as the security color and the contrast color.

use.

Figure 1 chromatic area map of safe color and contrast color

Table 6 Chromaticity and brightness factor of common materials, luminescent materials, retroreflective materials and composite materials

colour

Corner chromaticity coordinates of the allowable color range (standard illuminator D65, 2° field of view) Luminance factor beta Coordinate 1 2 3 4 ordinary material Illuminate material Retroreflective material a combination Material Type 1 Type 2 Red chi 0.735 0.681 0.579 0.655 >=0.07 >=0.03 >=0.655 >=0.655 >=0.655 y 0.265 0.239 0.341 0.345 chi 0.049 0.172 0.210 0.137 >=0.05 >=0.05 >=0.45 >=0.45 >=0.45 y 0.125 0.198 0.160 0.038 chi 0.545 0.494 0.444 0.481 >=0.45 >=0.80 >=0.05 >=0.05 >=0.05 y 0.454 0.426 0.476 0.518 chi 0.201 0.285 0.170 0.026 >=0.12 >=0.40 >=0.12 >=0.12 >=0.12 y 0.776 0.441 0.364 0.399 chi 0.350 0.305 0.295 0.340 >=0.75 >=1.0 >=0.340 >=0.340 >=0.340 y 0.360 0.315 0.325 0.370 chi 0.385 0.300 0.260 0.345 <=0.03 - - - -y 0.355 0.270 0.310 0.395 a Determine the type of retroreflective material based on the coefficient of retroreflection.

Table 7 Chromaticity coordinates of a smaller range of common materials and retroreflective materials in the chromaticity diagram

Corner chromaticity coordinates of the allowable color range (standard illuminator D65, 2° field of view) Common material Retroreflective material a Type 1 type 2 1 2 3 4 1 2 3 4 1 2 3 4 x 0.660 0.610 0.700 0.735 0.660 0.160 0.700 0.735 0.660 0.610 0.700 0.735 y 0.340 0.340 0.250 0.265 0.340 0.340 0.250 0.265 0.340 0.340 0.250 0.265 chi 0.140 0.160 0.160 0.140 0.130 0.160 0.160 0.130 0.130 0.160 0.160 0.130 Common material Retroreflective material a Type 1 type 2 1 2 3 4 1 2 3 4 1 2 3 4 y 0.140 0.140 0.160 0.160 0.086 0.086 0.120 0.120 0.090 0.090 0.140 0.140 x 0.494 0.470 0.493 0.525 0.494 0.470 0.493 0.525 0.494 0.470 0.513 0.545 y 0.505 0.480 0.457 0.477 0.505 0.480 0.457 0.477 0.505 0.480 0.437 0.454 x 0.230 0.260 0.260 0.230 0.110 0.150 0.150 0.110 0.110 0.170 0.170 0.110 y 0.440 0.440 0.470 0.470 0.415 0.415 0.454 0.454 0.415 0.415 0.500 0.500 x 0.305 0.335 0.325 0.295 0.305 0.335 0.325 0.295 0.305 0.335 0.325 0.295 y 0.315 0.345 0.355 0.325 0.315 0.345 0.355 0.325 0.315 0.345 0.355 0.325 a Determine the type of retroreflective material based on the coefficient of retroreflection.

5.2 Font Requirements

The font requirements are detailed below.

a) The writing font must be done. the font is neat, the strokes are clear, the interval is even, and the arrangement is neat. The height of the font (indicated by h) the size series is. 1.8 mm, 2.5 mm, 3.5 mm, 5 mm, 7 mm, 10 mm, 14 mm, 20 mm, and note.

You need to write a larger font whose font height should be increased by the ratio.

b) Chinese characters should be written in bold and should be stipulated in the Chinese Character Simplification Plan officially promulgated by the State Council of the People's Republic of China.

Simplified word. The height h of the Chinese character should not be less than 3.5 mm, and its word width is generally h/.

c) Other requirements shall comply with the provisions of GB...

......
This preview omits tables, figures, formulas and parts of the technical clauses. The complete document — 21 pages — is available in the English PDF.

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