SN/T 5334.8-2020Digital PCR detection method for genetically modified plant products - Part 8: Genetically modified sugar beet (English PDF)

Foreword

SN/T 5334-2020 "Digital PCR Detection Method for Genetically Modified Plant Products" is divided into 8 parts.

--Part 1: General requirements and definitions;

--Part 2: Genetically modified soybeans;

--Part 3: Genetically modified corn;

--Part 4: Genetically modified rape;

--Part 5: Genetically modified cotton;

--Part 6: Genetically Modified Potatoes;

--Part 7: Genetically modified alfalfa;

--Part 8: Genetically modified sugar beet.

This document is part 8 of SN/T 5334-2020.

This document was drafted in accordance with the rules given in GB/T 1.1-2020.

Certain contents of this document may involve patents. The issuing agency of this document is not responsible for identifying these patents.

This document was proposed and managed by the General Administration of Customs of the People's Republic of China.

1 Scope

This document specifies the quantitative detection method of genetically modified strains specific to imported and exported sugar beets by digital PCR (dPCR).

This document is suitable for the quantitative detection of genetically modified H7-1 strain-specific components in sugar beet. The lower limit of quantitative detection in this document is 2.8 copies Bay/µL, the lower limit of qualitative detection is 0.56 copies/µL.

2 Normative references

The contents of the following documents constitute the indispensable clauses of this document through normative references in the text. Among them, dated quotations Only the version corresponding to that date is applicable to this document; for undated references, the latest version (including all amendments) is applicable Used in this document.

SN/T 5334.1 Digital PCR Method for Quantitative Detection of Genetically Modified Products Part 1 General Requirements and Definitions

3 Terms and definitions

The terms and definitions defined in SN/T 5334.1 apply to this document.

4 Principles of the method

See SN/T 5334.1.

5 Main equipment and reagents

5.1 Endogenous gene and exogenous gene. the internal standard gene is the glutamine synthetase gene (GS); the exogenous sequence is specific to the H7-1 strain the cross-border sequence at the 5'end of the insertion site of the foreign gene. See Appendix A for the specific gene sequence of genetically modified sugar beet H7-1 strain, specificity See Table 1 for the primer probe sequences used for dPCR quantitative detection.

5.2 For other equipment and reagent requirements, please refer to SN/T 5334.1.

6 Method operation steps

7 Judgment and expression of results

See SN/T 5334.1 for calculation and judgment of results.

- Both the internal standard gene and the foreign gene in the test result meet the negative and blank quality control requirements, indicating that the sample has not been detected the standard GS gene in sugar beet is expressed as "the sugar beet component is not detected in this sample";

- In the test results, the internal standard gene result is positive, but the foreign gene meets the quality control requirements of negative and blank, said it indicates that the standard GS gene in sugar beet was detected in the sample, but no strain-specific sequence was detected, and the expression was "No transgenic - In the test results, both the internal standard gene and the exogenous gene meet the quality control requirements of the digital PCR test, indicating that the sample was transferred Ingredients, content is ×%".

8 Anti-pollution measures

The anti-pollution measures are specified in SN/T 5334.1.

9 Sample storage

For sample storage, see SN/T 5334.1.

Appendix A

(Informative appendix)