SN/T 5209-2020Quarantine and identification method of Walnut beetle (English PDF)

6.1 Equipment

Labels, finger tubes, petri dishes, tweezers, biological incubators, scissors, insect needles, scalpels, gauze, brushes, handheld magnification Mirror, screwdriver, chisel, stereo microscope, tertiary board, thick paper.

6.2 Reagents

Soaking liquid (glacial acetic acid. formalin. 95% ethanol. distilled water = 4.6.15.30), 75% ethanol, absolute ethanol, glycerol.

7.1 Detection

Special attention should be paid to the inspection of the wood from the epidemic area. First, carefully observe whether there are wormholes and wormholes on the surface of the log. The toughness of the victim wood the bond between the bark and the sapwood is usually loose. Use a chisel or an ax to cut the bark and observe whether the phloem and the sapwood are harmful.

Hazard status (see Appendix C). There is a mother tunnel with sub-tunnels on both sides. The larvae continue to dig holes and feed in the sub-tunnel to make the whole hazard Shaped like a feather. The distribution of its various insect states in the wood is. eggs are arranged in sequence on both sides of the mother tunnel, larvae, pupae and newly emerging adults are usually at the end of the subtunnel, when checking for insects, it is necessary to break the tree to the end along with the extension of the tunnel to catch the insects. And use a brush to gently Sweep away the moth powder to look for eggs.

For wooden boxes or pallets from the epidemic area, check the dunnage at the bottom of the wooden box, and focus on whether it has bark. If bark is found, snails can be used.

Cut off the cortex with a wire knife or chisel, and observe whether there are insects or insect passages on the joint surface of the phloem and the sapwood. If there is an insect passage, you can use a chisel to follow the tunnel.

Going excavation, various insect states can be found at the end of the tunnel. It should be recorded at any time, and the found insects should be sent to the laboratory for further identification.

Store the wood or wooden packaging that bore the beetles in a sealed container. Put the quarantine eggs, larvae, pupae or adults into ethanol-glycerin protection Store the liquid in a finger tube. If only live larvae are obtained on site, they can be directly brought back to the laboratory along with the damaged wood or wooden packaging, and record the interception.

Obtain the time, location, host, collector, etc., after artificial breeding and emergence into adults, then further identification.

7.2 Feeding

Bring the wood or wooden bag that is found to be damaged by larvae back to the laboratory, and put it in the insect cage to prevent the emergence of adult insects from escaping, at 24 °C ~ 26 °C Breed under temperature conditions, and then perform morphological identification after the emergence of adults.

8.1 Pin Insert Specimen

Adult specimens can be made as needle specimens. Cut thicker paper into small triangle or rectangular strips, stick the insect body on it, and puncture it with an insect needle Thick paper, and then insert it in the specimen box for storage. The label should be made of white hard paper, and the collection time, location, host and collection of the specimen should be recorded and other information.

8.2 Immersion specimen

Clean and tidy up the adults, larvae and other insect samples to be observed and identified. When there is dirt or powder on the body surface, use 75% ethanol or Soak in the prepared dipping solution. If molecular testing is required, it can be dipped in absolute ethanol and stored frozen.

9.1 Identification features

9.1.1 Identification characteristics of Corthylini adults

The forehead is usually male and female, and different types have different characteristics. The apex of the eye is slightly concave, with 1 to 5 segments of the whip of the antennae, if the hammer-like part is Flat, the front and back are the same. The base segments of the forefoot are adjacent, the pronotum is usually slanted and rough, and the outer edges are generally rounded or with edges A finely convex line with a unique groove on the side covered by the elytra or most of it is covered by the elytra. The leading edge of the elytra is usually slightly downwards towards the front end Inclined, the tibia is slender, rarely exceeding 3 teeth.

9.1.2 Identification characteristics of Pityophthorus adults

Small type, long cylindrical. Eye kidney shape. The side of the antennae hammer is flat, and the front is oblong, with 3 to 4 segments, and 5 segments of the whip of the antenna.

The forehead is flat, the forehead of the male is sparsely engraved, the hairs are sparse, and the length is uneven; the forehead of the female is finely engraved, and the hairs are soft and straight, even and dense.

The length and width of the pronotum and dorsal plate are approximately equal. The lateral and anterior edges are arched into a semicircle, and the posterior edge is straight and bordered; the front half of the dorsal plate is squamous Area, the latter half is the engraved dot area, with a smooth back midline. The surface of the elytra is flat and consists of a row of round engraved dots, gradually from the base of the wing to the end of the wing.

Thinner; only hairs on the wing surface without scales. The elytra oblique fin slits and the first grooves on both sides protrude into narrow ridges, and the first grooves on the slit side

1 The carved ditch sinks into a narrow longitudinal ditch. There are small particles on the outer edge of the ditch, and the circle is small and weak. They are arranged in longitudinal rows on the edge of the ditch. Some types of longitudinal grooves are relatively Shallow, the particles disappear.

The plate-shaped part of the anterior stomach is completely separated from the crop. The front edge of the plate-shaped part is straight or cut. There is no midline, teeth and end teeth, only a little short Straight transverse tooth line. The two parts of the slab are clearly separated, but there is no boundary line. The slab is longer than the slab part and consists of more chewable tablets. Chew the brush is much longer than the closed bristles, which have serrations on the outside.

Male external genitalia. the spiral filaments in the penis are as narrow as a sword; there are end pieces; the middle process is slightly shorter than the penis; the male base is ring-shaped; the abdominal needles are longer than the penis the sum with the middle protrusion, slender; whether the lateral protrusion is uncertain. Secondary sex characteristics are manifested in the forehead.

9.1.3 The distinguishing characteristics of Toxicola vulgaris the body is small, the length of the body is 1.5mm~1.9mm, about 2.8 times~3.1 times the width of the body, the body color of the female is yellowish brown, and the male is almost black.

(See Appendix B). There are embedded partitions between the first and second nodes of the hammer-shaped part of the antenna. The forehead is slightly lifted to the edge of the supraspinus, and the lateral edge protrudes into a semicircular shape.

The distance from the eye margin is 2 to 3 times the diameter of the small eye; the female's forehead is flat or slightly concave, the surface is smooth, the engraved spots are fine and well-proportioned, and the hairs are dense.

The hairs near the edges are longer (see Figure B.3(a)); the frontal surface of the male is wide and concave, with rough spots, short and sparse hairs, and less conspicuous (see attached Picture B.3(b) in Record B). The longest hair is about half the distance between the eyes.

Pronothorax. The length is about 1.10 times to 1.16 times the width, half of the lateral edge is straight from the base, and the front edge is arched into a wide semicircle. The front edge has About 18 saw teeth (see Appendix D). The top of the dorsal plate is located in the middle or anterior to the middle, and the front half is a squamous tumor area.

The 4 to 6 rows are clearly arranged in nearly concentric circles (see Figure E.1 in Appendix E), and the number of squamous tumors on the front edge of the dorsal plate exceeds 12 (see Appendix Record E Figure E.4), adjacent squamous tumors in the same row are often connected at the base, and often overlap near the dorsal midline. The back half of the back panel is the engraved area, the surface is gentle and smooth, and the engraved dots are slightly larger and dense. The bristles on the front surface of the dorsal plate are longer, arranged neatly with squamous tumors, and the bristles on the back are thinner.

Elytra. the length is about 1.8 to 1.9 times the width, about 1.7 times the length of the pronotum and dorsal plate, 3/4 of the lateral edge is straight from the base, and the end of the elytra is blunt and round (See Appendix E, Figure E.2). The elytra has a steep slope and larger engraved points, arranged in rows to form engraved grooves, of which the first groove is carved and the second groove is carved.

Shallower. The gap between the grooves is smooth, and the width is about 1 or 1.5 times that of the engraved groove; the first gap is very wide and bulges up obviously; the second gap the part is the same width as the first groove, and is smooth and slightly leathery; each of the first and third grooves has a row of very sparse engraved spots. Short and thin in the groove the bristles extend to the base of the elytra, and most of the bristles in the inter-sulcus are arranged sparsely on the base engraved groove of the elytra slope. Males 1st and 2nd There are 1 rows of small particles in the grooves.