SN/T 4675.9-2016Determination of diethylene glycol in wine for export--GC/MS method (English PDF)

Foreword

SN/T 4675 "Method for Analysis of Export Wine Quality" is divided into 30 sections.

---SN/T 4675.1 Determination of glycerol in wine exports Enzymatic;

---SN/T 4675.2 Determination of 2,3-butanediol in exported wines by gas chromatography;

---SN/T 4675.3 Determination of Stable Carbon Isotope Ratio of Ethanol in Export Wines;

---SN/T 4675.4 Determination of Lactic Acid in Export Wines Enzymatic;

---SN/T 4675.5 Determination of organic acids in exported wines by ion chromatography;

---SN/T 4675.6 Determination of glucose, fructose and sucrose in exported wines;

---SN/T 4675.7 Determination of acetaldehyde in exported wines by gas chromatography-mass spectrometry;

---SN/T 4675.8 Determination of 5-hydroxymethylfurfural in wines by liquid chromatography;

---SN/T 4675.9 Determination of diethylene glycol in exported wines by gas chromatography-mass spectrometry;

---SN/T 4675.10 Determination of ochratoxin A in wines for liquid chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.11 Determination of seven anthocyanins in wines exported by ultra-high performance liquid chromatography;

---SN/T 4675.12 Determination of Lysozyme in Export Wines by Liquid Chromatography;

---SN/T 4675.13 Determination of residues of 2,4,6-trichloroanisole in exported wines by gas chromatography-mass spectrometry;

---SN/T 4675.14 Determination of natamycin in wines for export - Liquid chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.15 Determination of salicylic acid, dehydroacetic acid and p-chlorobenzoic acid in wines by liquid chromatography;

---SN/T 4675.16 Determination of fumaric acid in wine exports by liquid chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.17 Determination of butyltin content in exported wines by gas chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.18 Determination of dithiocarbamate residues in exported wines. Headspace gas chromatography;

---SN/T 4675.19 Determination of sodium, magnesium, potassium, calcium, chromium, manganese, iron, copper, zinc, arsenic, selenium, silver, cadmium, and lead in wine exports;

---SN/T 4675.20 Determination of rare earth elements in wines exported by inductively coupled plasma mass spectrometry;

---SN/T 4675.21 Determination of soluble inorganic salts in exported wines by ion chromatography;

---SN/T 4675.22 Determination of total sulfur dioxide in wines by colorimetric method;

---SN/T 4675.23 Determination of ammonia nitrogen in wine and grape juice exported by continuous flow analyzer method;

---SN/T 4675.24 Determination of the export of the Folin-Shaugha index of the wine by spectrophotometry;

---SN/T 4675.25 Determination of color of exported wines CIE1976 (L*a*b*) color space method;

---SN/T 4675.26 Determination of export turbidity scattered light method;

---SN/T 4675.27 Determination of alkaline ash content of exported wines;

---SN/T 4675.28 Export of wine bacteria, mold and yeast count;

---SN/T 4675.29 Real-time fluorescent PCR assay for wine yeast extraction in wine exports;

---SN/T 4675.30 Bayer splice yeast assay for real-time fluorescence PCR in exported wines.

This section is Part 9 of SN/T 4675.

This section was drafted in accordance with the rules given in GB/T 1.1-2009.

This part is proposed and managed by the National Certification and Accreditation Regulatory Commission.

1 Scope

This part of SN/T 4675 specifies gas chromatography-mass spectrometric methods for the determination of diethylene glycol in wine.

This section applies to the determination of diethylene glycol in wine.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document.

GB/T 6682 Analysis Laboratory Water Specifications and Test Methods

3 Method summary

The sample was directly diluted with ethanol and then detected by gas chromatography-mass spectrometry. External standard method was used for quantification.

4 Reagents and materials

Unless otherwise stated, the reagents used were of analytical grade and water was a grade of water specified in GB/T 6682.

4.1 Ethanol. Chromatographically pure.

4.2 Diethylene glycol reference material (CAS No. 111-46-6, C4H10O3). Purity >= 99%.

4.3 Diethylene glycol standard stock solution. Accurately weigh 0.1 g (accurate to 0.1 mg) of diethylene glycol standard (4.2), dissolve in ethanol and bring to volume 100mL. The diethylene glycol stock solution concentration was 1000 mg/L. The standard stock solution can be stored for 90 days at 4°C~6°C.

4.4 Diethylene glycol standard intermediate solution. Accurately absorb 0.50 mL of diethylene glycol stock solution (4.3) and dilute to 10 mL with ethanol. This diethylene glycol standard the intermediate solution concentration is 50 mg/L. The standard stock solution can be stored for 90 days at 4°C~6°C.

4.5 Diethylene glycol standard working solution. Take the appropriate amount of diethylene glycol standard intermediate solution (4.4) with ethanol to prepare a concentration of 0.0mg/L, 0.20 mg/L, 0.40 mg/L, 1.00 mg/L, 2.00 mg/L, 10.0 mg/L, 20.0 mg/L of the standard series of solutions were used.

4.6 Filter. 0.22 µm, organic system.

5 Instruments and Equipment

5.1 Gas Chromatography-Mass Spectrometry (GC-MS) with Electron Impact Ion Source (EI).

5.2 Ultrasonic cleaners.

5.3 Vortex oscillator.

6 Sample preparation

6.1 calm wine. the sample is mixed into the clean container as a sample, sealed and marked, to be tested.