SN/T 4675.2-2016Determination of 2, 3-butanediol in wine for export--Gas chromatographic method (English PDF)

Foreword

SN/T 4675 "Method for Analysis of Export Wine Quality" is divided into 30 sections.

---SN/T 4675.1 Determination of glycerol in wine exports Enzymatic;

---SN/T 4675.2 Determination of 2,3-butanediol in exported wines by gas chromatography;

---SN/T 4675.3 Determination of Stable Carbon Isotope Ratio of Ethanol in Export Wines;

---SN/T 4675.4 Determination of Lactic Acid in Export Wines Enzymatic;

---SN/T 4675.5 Determination of organic acids in exported wines by ion chromatography;

---SN/T 4675.6 Determination of glucose, fructose and sucrose in exported wines;

---SN/T 4675.7 Determination of acetaldehyde in exported wines by gas chromatography-mass spectrometry;

---SN/T 4675.8 Determination of 5-hydroxymethylfurfural in wines by liquid chromatography;

---SN/T 4675.9 Determination of diethylene glycol in exported wines by gas chromatography-mass spectrometry;

---SN/T 4675.10 Determination of ochratoxin A in wines for liquid chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.11 Determination of seven anthocyanins in wines exported by ultra-high performance liquid chromatography;

---SN/T 4675.12 Determination of Lysozyme in Export Wines by Liquid Chromatography;

---SN/T 4675.13 Determination of residual 2,4,6-trichlorotoluene ether in exported wines;

---SN/T 4675.14 Determination of natamycin in wines for export - Liquid chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.15 Determination of salicylic acid, dehydroacetic acid and p-chlorobenzoic acid in wines by liquid chromatography;

---SN/T 4675.16 Determination of fumaric acid in wine exports by liquid chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.17 Determination of butyltin content in exported wines by gas chromatography-mass spectrometry/mass spectrometry;

---SN/T 4675.18 Determination of dithiocarbamate residues in exported wines. Headspace gas chromatography;

---SN/T 4675.19 Determination of sodium, magnesium, potassium, calcium, chromium, manganese, iron, copper, zinc, arsenic, selenium, silver, cadmium, and lead in wine exports;

---SN/T 4675.20 Determination of rare earth elements in wines exported by inductively coupled plasma mass spectrometry;

---SN/T 4675.21 Determination of soluble inorganic salts in exported wines by ion chromatography;

---SN/T 4675.22 Determination of total sulfur dioxide in wines by colorimetric method;

---SN/T 4675.23 Determination of ammonia nitrogen in wine and grape juice exported by continuous flow analyzer method;

---SN/T 4675.24 Determination of the export of the Folin-Shaugha index of the wine by spectrophotometry;

---SN/T 4675.25 Determination of color of exported wines CIE1976 (L*a*b*) color space method;

---SN/T 4675.26 Determination of export turbidity scattered light method;

---SN/T 4675.27 Determination of alkaline ash content of exported wines;

---SN/T 4675.28 Export of wine bacteria, mold and yeast count;

---SN/T 4675.29 Real-time fluorescent PCR assay for wine yeast extraction in wine exports;

---SN/T 4675.30 Bayer splice yeast assay for real-time fluorescence PCR in exported wines.

This section is Part 2 of SN/T 4675.

This section was drafted in accordance with the rules given in GB/T 1.1-2009.

1 Scope

This part of SN/T 4675 specifies the determination of 2,3-butanediol in wine by gas chromatography.

This section applies to the determination of 2,3-butanediol content in wine.

2 Normative references

The following documents are indispensable for the application of this document. For dated references, only dated versions apply to this article Pieces. For undated references, the latest version (including all amendments) applies to this document.

GB/T 6682 Analytical laboratory water specifications and test methods.

3 Method summary

The sample was purified on a graphitized carbon black column, diluted with ethanol, measured by a gas chromatography-hydrogen flame ionization detector, and quantified by an external standard method.

4 Reagents and materials

Unless otherwise specified, all reagents are of analytical grade and water is a grade of water specified in GB/T 6682.

4.1 Absolute ethanol (C2H6O). Chromatographically pure.

4.2 Ethanol solution (9 1, volume ratio). Accurately measure 450 mL of ethanol (4.1), add water to 50 mL, and mix.

4.3 Reference material. meso-2,3-butanediol standard, C4H10O2, CAS number. 5341-95-7, purity greater than 99%.

4.4 Standard stock solution (10.0 mg/mL). Accurately weigh the appropriate amount of standard material (4.3), and make a standard stock of 10.0 mg/mL with water.

Liquid preparation.

4.5 Standard Intermediate Solution (100.0mg/L). Accurately take 1.0mL standard stock solution (4.4), place in 100mL volumetric flask, add ethanol Dilute the solution (4.2) to the mark. A standard intermediate solution with a concentration of 100.0 mg/L was prepared.

4.6 standard working solution. accurately measure the standard intermediate solution (4.5) 0.1mL, 0.5mL, 1.0mL, 2.0mL, 3.0mL, 5.0mL in a 10 mL volumetric flask, dilute to the mark with an ethanol solution (4.2), and prepare 1.0 mg/L, 5.0 mg/L, 10.0 mg/L, and 20.0 mg/L, respectively.

30.0 mg/L, 50.0 mg/L standard working solution.

4.7 Graphitized carbon black column. 250 mg/3 mL.

5 Instruments and Equipment

5.1 Gas chromatograph equipped with a hydrogen flame ionization detector.

5.2 Analytical balance. The amount of sense is 0.1mg.

5.3 Solid phase extraction device.

5.4 Pipette. 1.0 mL.

5.5 Microporous membrane. 0.22 µm, organic system.